GenCrispr Cas9 Enzymes


GenCrispr/Cas9 Genome Editing Products

 

CRISPR/Cas9 technology is a powerful technique that has revolutionized the field of sequence-specific gene editing due to it's high precision, simplicity and flexibility. The CRISPR/Cas9 system consists of two major components: the Cas9 nuclease and the sequence-specific guide RNA (gRNA). They can form a very stable ribonucleoprotein (RNP) complex. Once a specific DNA region is matched by the gRNA carrying Cas9, the target DNA is cleaved with a double stranded break. Cas9 RNP complex can be introduced into cells directly and act immediately after it enters the cell without transcription and translation. By matching the gRNA sequence with different functional domains, the CRISPR/Cas9 system and its variants have been used for a broad range of gene editing applications (knock-out, knock-in, gene up/down, genome purification and visualization et. al.).

GenScript offers multiple kits to assemble your own Cas9 and gRNA constructs, as well as purified Cas9 enzymes for in vitro and in vivo use.


GenCrispr Cas9 Enzymes

DNA-free gene editing can be achieved by using purified Cas9 enzymes with gRNA and transfecting them directly into your cells or protoplasts of interest. GenCrispr Cas9 nuclease is the recombinant Streptococcus pyogenes Cas9 protein purified from E. coliand can be used for genome editing by inducing site-specific double stranded breaks in double stranded DNA. Cas9 protein forms a very stable ribonucleoprotein (RNP) complex with the guide RNA (gRNA) component of the CRISPR/Cas9 system. The RNP complex recognizes the target site by matching gRNA with the genomic DNA sequence and leads to DNA breaks within 3 bases from the NGG PAM (Protospacer Adjacent Motif). Presence of a nuclear localization signal (NLS) fusion with Cas9 enzyme ensures nuclear localization.

 


Enzyme activity validation guarantee


The GenCrispr Gene editing technology has been licensed-in from the Broad Institute. The activity of all GenCrispr Cas9 nucleases has been validated by in-vitro or in-vivo assays. The bioactivity data for all enzymes is included in the protocol document.

 

Key applications


Applications

Cas9 nuclease

Nuclear localization and in vivo gene-editing

Double Strand Break

·         GenCrispr Cas9-C-NLS nuclease

·         GenCrispr Cas9-N-NLS nuclease

·         GenCrispr NLS-Cas9-NLS nuclease

Single Strand Break

·         GenCrispr NLS-D10A-Cas9 Nickase

FACS/Fluorescence

·         GenCrispr NLS-Cas9-EGFP nuclease

In vitro target DNA cleavage

GenCrispr Cas9 nuclease

 

Enzyme


Cat. No.

Product Name

Z03386-50

GenCrispr Cas9 Nuclease - 50 μg (5×10μg)(0.2mg/ml)

Z03386-10

GenCrispr Cas9 Nuclease - 10 μg (0.2mg/ml)

Z03385-50

GenCrispr Cas9-C-NLS Nuclease - 50 μg (1mg/ml)

Z03388-50

GenCrispr Cas9-N-NLS Nuclease - 50 µg (1mg/ml)

Z03388-100

GenCrispr Cas9-N-NLS Nuclease - 100 µg (4mg/ml)

Z03389-50

GenCrispr NLS-Cas9-NLS Nuclease - 50 µg (1mg/ml)

Z03389-100

GenCrispr NLS-Cas9-NLS Nuclease - 100 µg (4mg/ml)

Z03390-50

GenCrispr NLS-Cas9-D10A Nickase - 50 µg (1mg/ml)

Z03390-100

GenCrispr NLS-Cas9-D10A Nickase - 100 µg (4mg/ml)

Z03390-10

GenCrispr NLS-Cas9-D10A Nickase - 10 µg (1mg/ml)

Z03393-50

GenCrispr NLS-Cas9-EGFP Nuclease - 50 µg (1mg/ml)

Z03393-100

GenCrispr NLS-Cas9-EGFP Nuclease - 100 µg (3mg/ml)

 

Kits


Cat. No.

Product Name

L00688-25

GenCrispr Mutation Detection Kit - 25 reactions

L00688-100

GenCrispr Mutation Detection Kit - 100 reactions

L00689-30

GenCrispr sgRNA Screening Kit - 30 reactions

L00689-100

GenCrispr sgRNA Screening Kit - 100 reactions

L00690-25

High-Efficiency gRNA-Cas9-GFP Plasmid (linear) Assembly Kit - 25 reactions

L00690-10

High-Efficiency gRNA-Cas9-GFP Plasmid (linear) Assembly Kit - 10 reactions

L00691-25

High-Efficiency gRNA-Cas9-Puro Plasmid (linear) Assembly Kit - 25 reactions

L00691-10

High-Efficiency gRNA-Cas9-Puro Plasmid (linear) Assembly Kit - 10 reactions

L00692-25

High-Efficiency gRNA-Cas9-GFP Plasmid Assembly Kit - 25 reactions

L00692-10

High-Efficiency gRNA-Cas9-GFP Plasmid Assembly Kit - 10 reactions

L00693-25

High-Efficiency gRNA-Cas9-Puro Plasmid Assembly Kit - 25 reactions

L00693-10

High-Efficiency gRNA-Cas9-Puro Plasmid Assembly Kit - 10 reactions

L00694-50

GenCrispr sgRNA Synthesis Kit - 50 reactions

L00694-20

GenCrispr sgRNA Synthesis Kit - 20 reactions

 

GenCrispr Transfection reagent - New!

 

Related Products

Cat. No.

Product Name

Z03396-250

GenCrispr T7 Endonuclease I - 250 U (10000 U/ml)

Z03396-1250

GenCrispr T7 Endonuclease I - 1250 U (10000 U/ml)


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