Cas9 Plasmids
GenCrispr/Cas9 Genome Editing Products
CRISPR/Cas9 technology is a powerful technique that has revolutionized the field of sequence-specific gene editing due to it's high precision, simplicity and flexibility. The CRISPR/Cas9 system consists of two major components: the Cas9 nuclease and the sequence-specific guide RNA (gRNA). They can form a very stable ribonucleoprotein (RNP) complex. Once a specific DNA region is matched by the gRNA carrying Cas9, the target DNA is cleaved with a double stranded break. Cas9 RNP complex can be introduced into cells directly and act immediately after it enters the cell without transcription and translation. By matching the gRNA sequence with different functional domains, the CRISPR/Cas9 system and its variants have been used for a broad range of gene editing applications (knock-out, knock-in, gene up/down, genome purification and visualization et. al.).
GenScript offers multiple kits to assemble your own Cas9 and gRNA constructs, as well as purified Cas9 enzymes for in vitro and in vivo use.
GenCRISPR / Cas9 Plasmids
CRISPR/Cas9 Plasmid Reagents for sgRNA Expression & Gene Editing
CRISPR/Cas9-mediated gene editing is a powerful technique that allows you to create knock-in/out mutations in any gene and any cell. CRISPR/Cas9 is advantageous over other forms of gene editing, such as TALENs and zinc finger nucleases, because it is simpler to implement and edits at higher efficiency.
GenScript licenses CRISPR technology from the Broad Institute of MIT and Harvard. Our offerings include the latest CRISPR plasmids and databases developed by the CRISPR pioneering Feng Zhang laboratory. Broad Institute-validated plasmids are a well-tested platform for expressing CRISPR/Cas9, and avoid instability issues in RNA-based platforms.
GenScript CRISPR Plasmid Repository
GenScript maintains a collection of more than 20,000 lentiCRISPRv2 plasmids containing guide RNA (gRNA) sequences pre-validated by the Broad Institute. Plasmids can be searched by gene name, symbol or ID on our gRNA Database.
Product |
Vector |
Selection |
GenCRISPR Plasmid Collection |
Lentiviral |
Amp, Puro |
Enhanced CRISPR/SpCas9 PlasmidsNew!
Enhanced specificity SpCas9 (eSpCas9), also referred to as SpCas9 (K848A/K1003A/R1060A), is structurally engineered for improved target specificity by researchers at the Feng Zhang Laboratory at the Broad Institute (Slaymaker et al. 2016). eSpCas9 is able to reduce off-target effects by over 10-fold, while maintaining robust on-target genome editing efficiency.
Product |
Vector |
Selection |
eSpCas9 Plasmids |
Plasmid |
Puro or GFP |
SpCas9 Plasmids
Cas9 endonuclease is the research standard for gene editing. When combined with single guide RNA (sgRNA) sequences, these enzymes create site-specific double strand breaks (DSBs) in the genome.
Product |
Vector |
Selection |
SpCas9 Plasmids |
Plasmid |
Amp |
SpCas9 Nickase Plasmids
SpCas9 nickase (Cas9n D10A) contains a mutation allowing the endonuclease to create single-strand nicks, as opposed to DSBs. Pairing two opposite facing gRNA sequences with SpCas9 nickase is an efficient method of gene editing that prevents unwanted indels from forming.
Product |
Vector |
Selection |
SpCas9 Nickase Plasmids |
Plasmid |
Amp |
SaCas9 Plasmids
The Staphylococcus aureus Cas9 orthologue (SaCas9) is the preferred endonuclease for adeno-associated virus (AAV) applications. SaCas9 is approximately 1kb shorter than SpCas9, and offers additional flexibility around AAV packaging constraints. The lower immunogenicity of AAV vectors, makes SaCas9 well-suited for in vivoediting applications and therapeutics.
Product |
Vector |
Selection |
SpCas9 Plasmids |
AVV |
Amp |
Transcription Activation (SAM) Plasmids
The CRISPR/Cas9 Synergistic Activation Mediator (SAM) system has been engineered to enable transcriptional activation of downstream targets. The SAM system utilizes three different activators, VP64, P65, and HSF1, which are assembled onto a catalytically dead Cas9 (dCas9) complex to drive transcription.
Product |
Vector |
Selection |
SAM gRNA Plasmids |
Plasmid |
Amp |
SAM dCas9-VP64 Plasmids |
Lentiviral |
Amp, Blast |
SAM MS2-P65-HSF1 Plasmids |
Lentiviral |
Amp, GFP |
Broad Institute Plasmid Collection
Broad Institute Plasmids are generated by the Broad Institute of Harvard and MIT. These plasmids contain a 17bp-1.8kb expressible linker in lieu of a customized sgRNA sequence, which can be modified by your laboratory.
Product |
Vector |
Selection |
SpCas9 |
Plasmid |
Amp, Puro |
SpCas9 Nickase |
Plasmid |
Amp |
SaCas9 |
AAV |
Amp |
CRISPR Vector Specifications
Service |
Vector |
Cas9/gRNA Expression |
Delivery Method |
Vector Type |
Selection Marker |
eSpCas9 Plasmids |
eSpCas9 & gRNA |
Plasmid |
All-in-one Vector |
AmpR |
|
eSpCas9 Plasmids |
eSpCas9 & gRNA |
Plasmid |
All-in-one Vector |
AmpR |
|
eSpCas9 Plasmids |
eSpCas9 & gRNA |
Lentiviral |
All-in-one Vector |
AmpR |
|
SpCas9 Plasmids |
SpCas9 & gRNA |
Plasmid |
All-in-one Vector |
AmpR |
|
SpCas9 Plasmids |
SpCas9 & gRNA |
Plasmid |
All-in-one Vector |
AmpR |
|
SpCas9 Plasmids |
SpCas9 & gRNA |
Lentiviral |
All-in-one Vector |
AmpR |
|
SpCas9 Plasmids |
gRNA Only |
Lentiviral |
Dual Vector |
AmpR |
|
SpCas9 Plasmids |
SpCas9 Only |
Lentiviral |
Dual Vector |
AmpR |
|
SpCas9 Plasmids |
SpCas9 Only |
Lentiviral |
Dual Vector |
AmpR |
|
SpCas9 Plasmids |
gRNA Only |
Plasmid |
Dual Vector |
AmpR |
|
SpCas9 Plasmids |
gRNA Only |
Plasmid |
Dual Vector |
AmpR |
|
SpCas9 Plasmids |
SpCas9 Only |
Plasmid |
Dual Vector |
AmpR |
|
SpCas9 Plasmids |
gRNA Only |
AAV |
Dual Vector |
AmpR |
|
SpCas9 Plasmids |
SpCas9 Only |
AAV |
Dual Vector |
AmpR |
|
SpCas9 Nickase Plasmids |
SpCas9 Nickase & gRNA |
Plasmid |
All-in-one Vector |
AmpR |
|
SpCas9 Nickase Plasmids |
SpCas9 Nickase & gRNA |
Plasmid |
All-in-one Vector |
AmpR |
|
SpCas9 Nickase Plasmids |
SpCas9 Nickase & gRNA |
Plasmid |
All-in-one Vector |
AmpR |
|
SpCas9 Nickase Plasmids |
SpCas9 Nickase Only |
Lentiviral |
Dual Vector |
AmpR |
|
SaCas9 Plasmids |
SaCas9 & gRNA |
AAV |
All-in-one Vector |
AmpR |
|
Transcription Activation (SAM) |
gRNA Only |
Plasmid |
SAM Multi Vector |
AmpR |
|
Transcription Activation (SAM) |
gRNA Only |
Lentiviral |
SAM Multi Vector |
AmpR |
|
Transcription Activation (SAM) |
Cas9 Activator |
Lentiviral |
SAM Multi Vector |
AmpR |
|
Transcription Activation (SAM) |
Cas9 Activator |
Lentiviral |
SAM Multi Vector |
AmpR |
|
Transcription Activation (SAM) |
Activator Adapter |
Lentiviral |
Multi Vector |
AmpR |
|
Transcription Activation (SAM) |
Activator Adapter |
Lentiviral |
Multi Vector |
AmpR |