Cas9 Plasmids


GenCrispr/Cas9 Genome Editing Products

 

CRISPR/Cas9 technology is a powerful technique that has revolutionized the field of sequence-specific gene editing due to it's high precision, simplicity and flexibility. The CRISPR/Cas9 system consists of two major components: the Cas9 nuclease and the sequence-specific guide RNA (gRNA). They can form a very stable ribonucleoprotein (RNP) complex. Once a specific DNA region is matched by the gRNA carrying Cas9, the target DNA is cleaved with a double stranded break. Cas9 RNP complex can be introduced into cells directly and act immediately after it enters the cell without transcription and translation. By matching the gRNA sequence with different functional domains, the CRISPR/Cas9 system and its variants have been used for a broad range of gene editing applications (knock-out, knock-in, gene up/down, genome purification and visualization et. al.).

GenScript offers multiple kits to assemble your own Cas9 and gRNA constructs, as well as purified Cas9 enzymes for in vitro and in vivo use.


GenCRISPR / Cas9 Plasmids


CRISPR/Cas9 Plasmid Reagents for sgRNA Expression & Gene Editing

CRISPR/Cas9-mediated gene editing is a powerful technique that allows you to create knock-in/out mutations in any gene and any cell. CRISPR/Cas9 is advantageous over other forms of gene editing, such as TALENs and zinc finger nucleases, because it is simpler to implement and edits at higher efficiency.

GenScript licenses CRISPR technology from the Broad Institute of MIT and Harvard. Our offerings include the latest CRISPR plasmids and databases developed by the CRISPR pioneering Feng Zhang laboratory. Broad Institute-validated plasmids are a well-tested platform for expressing CRISPR/Cas9, and avoid instability issues in RNA-based platforms.


GenScript CRISPR Plasmid Repository

GenScript maintains a collection of more than 20,000 lentiCRISPRv2 plasmids containing guide RNA (gRNA) sequences pre-validated by the Broad Institute. Plasmids can be searched by gene name, symbol or ID on our gRNA Database.

Product

Vector

Selection

GenCRISPR Plasmid Collection

Lentiviral

Amp, Puro

Enhanced CRISPR/SpCas9 PlasmidsNew!

Enhanced specificity SpCas9 (eSpCas9), also referred to as SpCas9 (K848A/K1003A/R1060A), is structurally engineered for improved target specificity by researchers at the Feng Zhang Laboratory at the Broad Institute (Slaymaker et al. 2016).  eSpCas9 is able to reduce off-target effects by over 10-fold, while maintaining robust on-target genome editing efficiency. 

 

Product

Vector

Selection

eSpCas9 Plasmids

Plasmid
Lentiviral

Puro or GFP

SpCas9 Plasmids

Cas9 endonuclease is the research standard for gene editing. When combined with single guide RNA (sgRNA) sequences, these enzymes create site-specific double strand breaks (DSBs) in the genome. 

Product

Vector

Selection

SpCas9 Plasmids

Plasmid
Lentiviral
AAV

Amp
Amp, Puro
Amp, Neo
Amp, GFP

SpCas9 Nickase Plasmids

SpCas9 nickase (Cas9n D10A) contains a mutation allowing the endonuclease to create single-strand nicks, as opposed to DSBs. Pairing two opposite facing gRNA sequences with SpCas9 nickase is an efficient method of gene editing that prevents unwanted indels from forming.

Product

Vector

Selection

SpCas9 Nickase Plasmids

Plasmid
Lentiviral

Amp
Amp, Puro
Amp, GFP

SaCas9 Plasmids

The Staphylococcus aureus Cas9 orthologue (SaCas9) is the preferred endonuclease for adeno-associated virus (AAV) applications. SaCas9 is approximately 1kb shorter than SpCas9, and offers additional flexibility around AAV packaging constraints. The lower immunogenicity of AAV vectors, makes SaCas9 well-suited for in vivoediting applications and therapeutics. 

 

Product

Vector

Selection

SpCas9 Plasmids

AVV

Amp

Transcription Activation (SAM) Plasmids

The CRISPR/Cas9 Synergistic Activation Mediator (SAM) system has been engineered to enable transcriptional activation of downstream targets. The SAM system utilizes three different activators, VP64, P65, and HSF1, which are assembled onto a catalytically dead Cas9 (dCas9) complex to drive transcription. 

 

Product

Vector

Selection

SAM gRNA Plasmids

Plasmid
Lentiviral

Amp
Amp, Zeo

SAM dCas9-VP64 Plasmids

Lentiviral

Amp, Blast
Amp, GFP

SAM MS2-P65-HSF1 Plasmids

Lentiviral

Amp, GFP
Amp, Hygro

Broad Institute Plasmid Collection

Broad Institute Plasmids are generated by the Broad Institute of Harvard and MIT. These plasmids contain a 17bp-1.8kb expressible linker in lieu of a customized sgRNA sequence, which can be modified by your laboratory.

Product

Vector

Selection

SpCas9
Broad Plasmid Collection

Plasmid
Lentiviral

Amp, Puro
Amp, GFP

SpCas9 Nickase
Broad Plasmid Collection

Plasmid

Amp
Amp, Puro
Amp, GFP

SaCas9
Broad Plasmid Collection

AAV

Amp


CRISPR Vector Specifications


Service

Vector

Cas9/gRNA Expression

Delivery Method

Vector Type

Selection Marker

eSpCas9 Plasmids

eSpCas9-2A- GFP (PX458) New!

eSpCas9 & gRNA

Plasmid

All-in-one Vector

AmpR
EGFP

eSpCas9 Plasmids

eSpCas9-2A- Puro (PX459) V2.0 New!

eSpCas9 & gRNA

Plasmid

All-in-one Vector

AmpR
PuroR

eSpCas9 Plasmids

eSpCas9-LentiCRISPR v2 New!

eSpCas9 & gRNA

Lentiviral

All-in-one Vector

AmpR
PuroR

SpCas9 Plasmids

pSpCas9 BB-2A-GFP PX458

SpCas9 & gRNA

Plasmid

All-in-one Vector

AmpR
EGFP

SpCas9 Plasmids

pSpCas9 BB-2A-Puro (PX459) v2.0

SpCas9 & gRNA

Plasmid

All-in-one Vector

AmpR
PuroR

SpCas9 Plasmids

pLentiCRISPR v2

SpCas9 & gRNA

Lentiviral

All-in-one Vector

AmpR
PuroR

SpCas9 Plasmids
SpCas9 Nickase Plasmids

pLentiGuide-Puro

gRNA Only

Lentiviral

Dual Vector

AmpR
PuroR

SpCas9 Plasmids

pLentiCas9-Blast

SpCas9 Only

Lentiviral

Dual Vector

AmpR
BsdR
BleoR

SpCas9 Plasmids

pLentiCas9-EGFP

SpCas9 Only

Lentiviral

Dual Vector

AmpR
EGFP

SpCas9 Plasmids

pGS-gRNA

gRNA Only

Plasmid

Dual Vector

AmpR

SpCas9 Plasmids

pGS-gRNA-Neo

gRNA Only

Plasmid

Dual Vector

AmpR
NeoR

SpCas9 Plasmids

pSpCas9 PX165

SpCas9 Only

Plasmid

Dual Vector

AmpR

SpCas9 Plasmids

pAAV_SpGuide acceptor (PX552)

gRNA Only

AAV

Dual Vector

AmpR
EGFP

SpCas9 Plasmids

pAAV-SpCas9 PX551

SpCas9 Only

AAV

Dual Vector

AmpR

SpCas9 Nickase Plasmids

pSpCas9n BB PX460

SpCas9 Nickase & gRNA

Plasmid

All-in-one Vector

AmpR

SpCas9 Nickase Plasmids

pSpCas9n BB-2A-GFP PX461

SpCas9 Nickase & gRNA

Plasmid

All-in-one Vector

AmpR
EGFP

SpCas9 Nickase Plasmids

pSpCas9n BB-2A-Puro (PX462) V2.0

SpCas9 Nickase & gRNA

Plasmid

All-in-one Vector

AmpR
PuroR

SpCas9 Nickase Plasmids

pLentiCas9n-Blast

SpCas9 Nickase Only

Lentiviral

Dual Vector

AmpR
BsdR
BleoR

SaCas9 Plasmids

pX601_AAV

SaCas9 & gRNA

AAV

All-in-one Vector

AmpR

Transcription Activation (SAM)

pSgRNA(MS2)

gRNA Only

Plasmid

SAM Multi Vector

AmpR

Transcription Activation (SAM)

pLenti_sgRNA(MS2)_zeo

gRNA Only

Lentiviral

SAM Multi Vector

AmpR
ZeoR
BeloR

Transcription Activation (SAM)

pLenti_dCas9-VP64_Blast

Cas9 Activator

Lentiviral

SAM Multi Vector

AmpR
BlastR
BleoR

Transcription Activation (SAM)

pLenti_dCas9-VP64_GFP

Cas9 Activator

Lentiviral

SAM Multi Vector

AmpR
EGFP
BleoR

Transcription Activation (SAM)

pLenti_MS2-P65-HSF1_Hygro

Activator Adapter

Lentiviral

Multi Vector

AmpR
HygroR
BleoR

Transcription Activation (SAM)

pLenti_MS2-P65-HSF1_GFP

Activator Adapter

Lentiviral

Multi Vector

AmpR
EGFP
BleoR


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