Secondary Antibodies
Secondary Antibodies
A primary antibody's Fc domain is constant within the same animal species, therefore, a given secondary antibody can be used with any primary antibody of the same isotype and target species. This reduces the cost by labeling only one type of secondary antibody. In addition, secondary antibodies offer increased sensitivity through the signal amplification.
At GenScript, secondary antibodies are purified from the serum of several hosts, including goat, donkey, mouse, rabbit, chicken and sheep. Secondary antibodies are available with specificity for whole Ig molecules or antibody fragments such as the Fc or Fab regions. The conjugated secondary antibodies can be used for various immunology analysis and protein assays.
GenScript also offers over 1000 highly specific monoclonal and polyclonal primary antibodies.
<<Featured Products>>
MonoRabᵀᴹ Rabbit Anti-Camelid VHH Monoclonal Antibody Series
Camelids naturally produce antibodies composed only of heavy chains as compared to the conventional antibodies (Fig 1)The antigen-binding site of these unusual heavy chain antibodies is formed only by a single heavy chain variable domain, designated as single domain antibodies (sdAbs or VHHs). Because of their unique structure, small size and stability, VHHs have several advantages comparing to conventional antibodies or their fragments. Several VHHs are now being studied for use in the diagnostic fields and therapeutic areas, including oncology, infectious, inflammatory, and neurodegenerative diseases.
Rabbit anti-camelid VHH monoclonal antibody series generated by Genscript's proprietary rabbit-mouse hetero-hybridoma technology MonoRab™ provide perfect secondary antibody tools for researchers to identify target-specific sdAbs.
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Cat. No. |
Product Name |
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A01860-200 |
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A01995-200 |
MonoRabᵀᴹ Rabbit Anti-Camelid VHH Antibody [Biotin], mAb - 200 µl |
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A01861-200 |
MonoRabᵀᴹ Rabbit Anti-Camelid VHH Antibody [HRP], mAb - 200 µl |
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A01862-200 |
MonoRabᵀᴹ Rabbit Anti-Camelid VHH Antibody [iFluor488], mAb - 200 µl |
|
A01863-200 |
MonoRabᵀᴹ Rabbit Anti-Camelid VHH Antibody [iFluor555], mAb - 200 µl |
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A01994-200 |
MonoRabᵀᴹ Rabbit Anti-Camelid VHH Antibody [iFluor 647], mAb-200µl |
Key Features
- MonoRab™ technology guarantees high affinity: Kd ≈ 10-11 M
- No cross reactivity with other species
- Recognizes the variable domain of camelid (Llama and Camel) antibodies
- Specific to camelid IgG2 & IgG3
- Recognizes conformational epitope
Mouse Anti-Rabbit IgG Antibody (M205) [HRP], mAb
Conventional polyclonal antibodies recognize many epitopes, leading to high background and cross reactivity in many assays. M205 is specific to native rabbit IgG and doesn't react with denatured or reduced rabbit IgG heavy or light chain. It's an ideal tool for Immunoprecipitation and Western blot. Primary antibody heavy and light chain contamination can be avoided.
The result demonstrates that Anti-Rabbit IgG Monoclonal Secondary Antibody (M205) provides less background noise than conventional polyclonal secondary antibody with equivalent sensitivity.
Detection of different titrated primary rabbit antibodies using Anti-Rabbit IgG Monoclonal Secondary Antibody (M205) (HRP conjugate)#A1827 indicates that M205 binds to a conserved rabbit IgG epitope, and can recognize primary antibodies generated from different rabbits.
All Products
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Goat |
Rabbit IgG (H&L) |
Peroxidase (Horseradish) |
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Goat |
Rabbit IgG (H&L) |
Unconjugated |
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Goat |
Mouse IgG (H&L) |
Peroxidase (Horseradish) |
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Goat |
Chicken IgY (H&L) |
Peroxidase (Horseradish) |
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Goat |
Human IgG (H&L) |
Peroxidase (Horseradish) |
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Goat |
Rat IgG (H&L) |
Peroxidase (Horseradish) |
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Donkey |
Goat IgG |
Peroxidase (Horseradish) |
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Goat |
Mouse IgG (H&L) |
Fluorescein (FITC) |
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Mouse |
Rabbit IgG |
Peroxidase (Horseradish) |
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Mouse |
Human IgG F(c) |
Peroxidase (Horseradish) |
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Mouse |
Human IgG F(ab) |
Peroxidase (Horseradish) |
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Mouse |
Rabbit IgG F(c) |
Peroxidase (Horseradish) |
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Rabbit |
Camelid VHH |
Unconjugated |
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Rabbit |
Camelid VHH |
Peroxidase (Horseradish) |
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Rabbit |
Camelid VHH |
iFluor488 |
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Rabbit |
Camelid VHH |
iFluor555 |
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Goat |
Mouse IgM (H&L) |
Unconjugated |
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Rabbit |
Mouse IgM (H&L) |
Unconjugated |
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Goat |
Mouse IgM (H&L) |
Fluorescein (FITC) |
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Rabbit |
Mouse IgM (H&L) |
Unconjugated |
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Rabbit |
Mouse IgM (H&L) |
Fluorescein (FITC) |
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Rabbit |
Mouse IgM (H&L) |
Peroxidase (Horseradish) |
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Rabbit |
Bovine IgG F(ab')2 |
Texas Red® |
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Goat |
Chicken IgG (H&L) |
Texas Red® |
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Rabbit |
Chicken IgG (H&L) |
Texas Red® |
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Goat |
Dog IgG F(c) |
Texas Red® |
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Rabbit |
Goat IgG F(c) |
Peroxidase (Horseradish) |
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Rabbit |
Goat IgG (H&L) |
Texas Red® |
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Donkey |
Goat IgG (H&L) |
Peroxidase (Horseradish) |
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Donkey |
Goat IgG (H&L) |
Alkaline Phosphatase (Calf Intestine) |
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Donkey |
Goat IgG (H&L) |
Biotin |
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Donkey |
Goat IgG (H&L) |
Texas Red® |
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Goat |
Guinea Pig IgG (H&L) |
Texas Red® |
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Goat |
Human IgG F(c) |
Unconjugated |
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Goat |
Human IgG (H&L) |
Unconjugated |
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Goat |
Human IgG (H&L) |
Fluorescein (FITC) |
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Goat |
Human IgG F(c) |
Peroxidase (Horseradish) |
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Goat |
Human IgG (H&L) |
Peroxidase (Horseradish) |
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Goat |
Human IgG IgA IgM |
Peroxidase (Horseradish) |
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Goat |
Human IgG (H&L) |
Alkaline Phosphatase (Calf Intestine) |
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Goat |
Human IgG IgA IgM |
Alkaline Phosphatase (Calf Intestine) |
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Goat |
Human IgG F(c) |
Biotin |
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Goat |
Human IgG (H&L) |
Biotin |
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Goat |
Human IgG (H&L) |
R-Phycoerythrin (RPE) |
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Goat |
Human IgG (H&L) |
Unconjugated |
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Goat |
Human IgG F(c) |
Unconjugated |
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Goat |
Human IgG F(ab')2 |
Unconjugated |
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Goat |
Human IgM µ chain |
Unconjugated |
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Goat |
Human κ chain |
Unconjugated |
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Goat |
Human λ chain |
Unconjugated |
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Goat |
Human IgG (H&L) |
Unconjugated |
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Goat |
Human IgG (H&L) |
Fluorescein (FITC) |
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Goat |
Human IgG (H&L) |
Peroxidase (Horseradish) |
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Goat |
Human IgG F(c) |
Peroxidase (Horseradish) |
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Goat |
Human IgG F(ab')2 |
Peroxidase (Horseradish) |
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Goat |
Human IgM µ chain |
Peroxidase (Horseradish) |
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Goat |
Human IgG (H&L) |
Peroxidase (Horseradish) |
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Goat |
Human IgG F(ab')2 |
Biotin |
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Goat |
Human κ chain |
Biotin |
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Goat |
Human IgG (H&L) |
Texas Red® |
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Mouse |
Human IgG (H&L) |
Unconjugated |
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Rabbit |
Human IgG (H&L) |
Unconjugated |
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Rabbit |
Human IgA |
Unconjugated |
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Rabbit |
Human IgG F(ab')2 |
Unconjugated |
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Rabbit |
Human IgG (H&L) |
Peroxidase (Horseradish) |
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Rabbit |
Human IgG F(c) |
Peroxidase (Horseradish) |
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Rabbit |
Human IgG (γ chain) |
Peroxidase (Horseradish) |
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Rabbit |
Human IgG (H&L) |
Peroxidase (Horseradish) |
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Rabbit |
Human IgG F(ab')2 |
Peroxidase (Horseradish) |
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Rabbit |
Human IgM Fc5µ |
Peroxidase (Horseradish) |
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Rabbit |
Human IgG (H&L) |
Alkaline Phosphatase (Calf Intestine) |
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Rabbit |
Human IgG F(c) |
Alkaline Phosphatase (Calf Intestine) |
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Rabbit |
Human IgG F(c) |
Biotin |
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Rabbit |
Human IgA |
Biotin |
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Rabbit |
Human IgG (H&L) |
Texas Red® |
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Rabbit |
Human IgG F(c) |
Texas Red® |
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Rabbit |
Human IgA |
Texas Red® |
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Sheep |
Human IgG (H&L) |
Peroxidase (Horseradish) |
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Sheep |
Human IgG (H&L) |
Biotin |
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Chicken |
Human IgG (H&L) |
Peroxidase (Horseradish) |
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Chicken |
Human IgG (H&L) |
Texas Red® |
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Goat |
Mouse IgG + IgM (H&L) |
Rhodamine (TRITC) |
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Goat |
Mouse IgG (H&L) |
Rhodamine (TRITC) |
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Goat |
Mouse IgG (H&L) |
Rhodamine (TRITC) |
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Goat |
Mouse IgG (H&L) |
Unconjugated |
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Goat |
Mouse IgG IgA IgM |
Unconjugated |
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Goat |
Mouse IgG + IgM (H&L) |
Fluorescein (FITC) |
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Goat |
Mouse IgG (H&L) |
Fluorescein (FITC) |
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Goat |
Mouse IgG1 |
Peroxidase (Horseradish) |
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Goat |
Mouse IgG2b |
Peroxidase (Horseradish) |
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Goat |
Mouse IgG + IgM (H&L) |
Peroxidase (Horseradish) |
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Goat |
Mouse IgG (H&L) |
Peroxidase (Horseradish) |
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Goat |
Mouse IgG IgA IgM |
Peroxidase (Horseradish) |
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Goat |
Mouse IgG IgA IgM |
Peroxidase (Horseradish) |
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Goat |
Mouse IgG (H&L) |
Alkaline Phosphatase (Calf Intestine) |
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Goat |
Mouse IgG (H&L) |
Biotin |
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Goat |
Mouse IgG (H&L) |
R-Phycoerythrin (RPE) |
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Goat |
Mouse IgG (H&L) |
Texas Red® |
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Goat |
Mouse IgG (H&L) |
Unconjugated |
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Goat |
Mouse IgG F(c) |
Unconjugated |
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Goat |
Mouse IgM µ chain |
Unconjugated |
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Goat |
Mouse IgG (H&L) |
Unconjugated |
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Goat |
Mouse IgG (H&L) |
Fluorescein (FITC) |
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Goat |
Mouse IgG F(ab')2 |
Fluorescein (FITC) |
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Goat |
Mouse IgG (H&L) |
Peroxidase (Horseradish) |
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Goat |
Mouse IgG F(c) |
Peroxidase (Horseradish) |
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Goat |
Mouse IgG F(ab')2 |
Peroxidase (Horseradish) |
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Goat |
Mouse IgG (H&L) |
Peroxidase (Horseradish) |
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Goat |
Mouse IgG (H&L) |
Alkaline Phosphatase (Calf Intestine) |
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Goat |
Mouse IgG (H&L) |
Alkaline Phosphatase (Calf Intestine) |
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Goat |
Mouse IgG (H&L) |
Biotin |
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Goat |
Mouse IgG (H&L) |
Texas Red® |
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Rabbit |
Mouse IgG (H&L) |
Rhodamine (TRITC) |
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Rabbit |
Mouse κ chain |
Rhodamine (TRITC) |
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Rabbit |
Mouse IgG (H&L) |
Unconjugated |
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Rabbit |
Mouse IgG F(c) |
Unconjugated |
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Rabbit |
Mouse IgM µ chain |
Unconjugated |
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Rabbit |
Mouse κ chain |
Unconjugated |
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Rabbit |
Mouse λ chain |
Unconjugated |
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Rabbit |
Mouse IgG (γ chain) |
Unconjugated |
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Rabbit |
Mouse IgG (H&L) |
Unconjugated |
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Rabbit |
Mouse IgG2b |
Unconjugated |
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Rabbit |
Mouse IgG3 |
Unconjugated |
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Rabbit |
Mouse IgG (H&L) |
Fluorescein (FITC) |
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Rabbit |
Mouse IgG1 |
Fluorescein (FITC) |
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Rabbit |
Mouse IgG2a |
Fluorescein (FITC) |
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Rabbit |
Mouse IgG F(c) |
Peroxidase (Horseradish) |
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Rabbit |
Mouse κ chain |
Peroxidase (Horseradish) |
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Rabbit |
Mouse IgG (γ chain) |
Peroxidase (Horseradish) |
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Rabbit |
Mouse IgG (H&L) |
Peroxidase (Horseradish) |
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Rabbit |
Mouse IgG2b |
Peroxidase (Horseradish) |
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Rabbit |
Mouse IgG3 |
Peroxidase (Horseradish) |
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Rabbit |
Mouse IgG (H&L) |
Alkaline Phosphatase (Calf Intestine) |
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Rabbit |
Mouse IgA |
Alkaline Phosphatase (Calf Intestine) |
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Rabbit |
Mouse κ chain |
Alkaline Phosphatase (Calf Intestine) |
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Rabbit |
Mouse λ chain |
Alkaline Phosphatase (Calf Intestine) |
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Rabbit |
Mouse IgG (H&L) |
Alkaline Phosphatase (Calf Intestine) |
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Rabbit |
Mouse IgG1 |
Alkaline Phosphatase (Calf Intestine) |
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Rabbit |
Mouse IgG2a |
Alkaline Phosphatase (Calf Intestine) |
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Rabbit |
Mouse IgG (H&L) |
Biotin |
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Rabbit |
Mouse IgG F(c) |
Biotin |
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Rabbit |
Mouse IgM µ chain |
Biotin |
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Rabbit |
Mouse IgG1 |
Biotin |
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Rabbit |
Mouse IgG2a |
Biotin |
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Rabbit |
Mouse IgG F(c) |
Texas Red® |
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Rabbit |
Mouse IgA |
Texas Red® |
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Rabbit |
Mouse IgG (γ chain) |
Texas Red® |
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Sheep |
Mouse IgG (H&L) |
Fluorescein (FITC) |
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Sheep |
Mouse IgG (H&L) |
Texas Red® |
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Donkey |
Mouse IgG (H&L) |
Unconjugated |
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Donkey |
Mouse IgG (H&L) |
Fluorescein (FITC) |
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Donkey |
Mouse IgG (H&L) |
Peroxidase (Horseradish) |
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Donkey |
Mouse IgG (H&L) |
Biotin |
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Donkey |
Mouse IgG (H&L) |
Texas Red® |
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Donkey |
Mouse IgG (H&L) |
Texas Red® |
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Chicken |
Mouse IgG (H&L) |
Unconjugated |
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Chicken |
Mouse IgG (H&L) |
Peroxidase (Horseradish) |
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Goat |
Rabbit IgG (H&L) |
Rhodamine (TRITC) |
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Goat |
Rabbit IgG (H&L) |
Unconjugated |
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Goat |
Rabbit IgG (H&L) |
Fluorescein (FITC) |
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Goat |
Rabbit IgG F(c) |
Rhodamine (TRITC) |
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Goat |
Rabbit IgG (H&L) |
Peroxidase (Horseradish) |
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Goat |
Rabbit IgG (H&L) |
Alkaline Phosphatase (Calf Intestine) |
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Goat |
Rabbit IgG (H&L) |
Biotin |
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Goat |
Rabbit IgG (H&L) |
Unconjugated |
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Goat |
Rabbit IgG F(c) |
Unconjugated |
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Goat |
Rabbit IgG (H&L) |
Unconjugated |
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Goat |
Rabbit IgG F(c) |
Unconjugated |
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Goat |
Rabbit IgG (H&L) |
Fluorescein (FITC) |
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Goat |
Rabbit IgG (H&L) |
Fluorescein (FITC) |
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Goat |
Rabbit IgG (H&L) |
Peroxidase (Horseradish) |
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Goat |
Rabbit IgG F(c) |
Peroxidase (Horseradish) |
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Goat |
Rabbit IgG F(ab')2 |
Peroxidase (Horseradish) |
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Goat |
Rabbit IgG (H&L) |
Peroxidase (Horseradish) |
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Goat |
Rabbit IgG F(c) |
Peroxidase (Horseradish) |
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Goat |
Rabbit IgG (H&L) |
Alkaline Phosphatase (Calf Intestine) |
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Goat |
Rabbit IgG F(c) |
Alkaline Phosphatase (Calf Intestine) |
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Goat |
Rabbit IgG (H&L) |
Alkaline Phosphatase (Calf Intestine) |
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Goat |
Rabbit IgG F(c) |
Alkaline Phosphatase (Calf Intestine) |
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Goat |
Rabbit IgG (H&L) |
Biotin |
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Goat |
Rabbit IgG F(c) |
Biotin |
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Goat |
Rabbit IgG F(c) |
Biotin |
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Goat |
Rabbit IgG (H&L) |
Texas Red® |
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Goat |
Rabbit IgG F(c) |
Texas Red® |
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Goat |
Rabbit IgG F(c) |
Texas Red® |
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Mouse |
Rabbit IgG (H&L) |
Unconjugated |
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Sheep |
Rabbit IgG (H&L) |
Peroxidase (Horseradish) |
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Donkey |
Rabbit IgG (H&L) |
Rhodamine (TRITC) |
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Donkey |
Rabbit IgG F(c) |
Unconjugated |
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Donkey |
Rabbit IgG (H&L) |
Unconjugated |
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Donkey |
Rabbit IgG F(c) |
Fluorescein (FITC) |
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Donkey |
Rabbit IgG (H&L) |
Fluorescein (FITC) |
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Donkey |
Rabbit IgG F(c) |
Peroxidase (Horseradish) |
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Donkey |
Rabbit IgG (H&L) |
Peroxidase (Horseradish) |
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Donkey |
Rabbit IgG (H&L) |
Alkaline Phosphatase (Calf Intestine) |
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Donkey |
Rabbit IgG F(c) |
Biotin |
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Donkey |
Rabbit IgG (H&L) |
Biotin |
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Donkey |
Rabbit IgG (H&L) |
Texas Red® |
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Donkey |
Rabbit IgG (H&L) |
Fluorescein (FITC) |
|
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Donkey |
Rabbit IgG (H&L) |
Peroxidase (Horseradish) |
|
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Donkey |
Rabbit IgG (H&L) |
Alkaline Phosphatase (Calf Intestine) |
|
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Donkey |
Rabbit IgG (H&L) |
Biotin |
|
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Donkey |
Rabbit IgG (H&L) |
Texas Red® |
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Chicken |
Rabbit IgG (H&L) |
Peroxidase (Horseradish) |
|
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Goat |
Rat IgG (H&L) |
Unconjugated |
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Goat |
Rat IgG (H&L) |
Peroxidase (Horseradish) |
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Goat |
Rat IgG (H&L) |
Biotin |
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Goat |
Rat IgG (H&L) |
R-Phycoerythrin (RPE) |
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Goat |
Rat IgG F(c) |
Unconjugated |
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Goat |
Rat IgM µ chain |
Unconjugated |
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Goat |
Rat IgG (H&L) |
Fluorescein (FITC) |
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Goat |
Rat IgG F(c) |
Fluorescein (FITC) |
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Goat |
Rat IgG (H&L) |
Peroxidase (Horseradish) |
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Goat |
Rat IgG F(c) |
Peroxidase (Horseradish) |
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Goat |
Rat IgM µ chain |
Peroxidase (Horseradish) |
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Goat |
Rat IgG (H&L) |
Alkaline Phosphatase (Calf Intestine) |
|
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Goat |
Rat IgG (H&L) |
Biotin |
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Goat |
Rat IgG F(c) |
Biotin |
|
|
Rabbit |
Rat IgG (H&L) |
Unconjugated |
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Rabbit |
Rat IgG (H&L) |
Fluorescein (FITC) |
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Rabbit |
Rat IgG (H&L) |
Peroxidase (Horseradish) |
|
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Rabbit |
Rat IgG (H&L) |
Biotin |
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Rabbit |
Rat IgG (H&L) |
Texas Red® |
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Sheep |
Rat IgG (H&L) |
Unconjugated |
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Donkey |
Rat IgG (H&L) |
Unconjugated |
|
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Donkey |
Rat IgG (H&L) |
Peroxidase (Horseradish) |
|
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Donkey |
Rat IgG (H&L) |
Texas Red® |
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Rabbit |
Sheep IgG (H&L) |
Texas Red® |
|
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Goat |
Swine IgG (H&L) |
Texas Red® |
|
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Goat |
Monkey IgA |
Unconjugated |
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Goat |
Monkey IgG (γ chain) |
Unconjugated |
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Goat |
Monkey IgG IgA IgM |
Unconjugated |
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Goat |
Monkey IgA |
Fluorescein (FITC) |
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Goat |
Monkey IgG (γ chain) |
Fluorescein (FITC) |
|
|
Goat |
Monkey IgA |
Peroxidase (Horseradish) |
|
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Goat |
Monkey IgM µ chain |
Peroxidase (Horseradish) |
|
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Goat |
Monkey IgG (γ chain) |
Peroxidase (Horseradish) |
|
|
Goat |
Monkey IgA |
Biotin |
|
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Goat |
Monkey IgG (γ chain) |
Biotin |
|
|
Goat |
Armenian Hamster IgG (H&L) |
Peroxidase (Horseradish) |
|
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Donkey |
Goat IgG (H&L) |
Peroxidase (Horseradish) |
|
|
Donkey |
Goat IgG (H&L) |
R-Phycoerythrin (RPE) |
|
|
Goat |
Human IgG IgA IgM |
Unconjugated |
|
|
Goat |
Human IgG IgA IgM |
Peroxidase (Horseradish) |
|
|
Goat |
Human IgG IgA IgM |
Alkaline Phosphatase (Calf Intestine) |
|
|
Goat |
Human IgA |
Unconjugated |
|
|
Goat |
Human κ chain |
Unconjugated |
|
|
Goat |
Human IgG (H&L) |
Unconjugated |
|
|
Goat |
Human IgG F(c) |
Unconjugated |
|
|
Goat |
Human κ chain |
Fluorescein (FITC) |
|
|
Goat |
Human λ chain |
Fluorescein (FITC) |
|
|
Goat |
Human IgA |
Peroxidase (Horseradish) |
|
|
Goat |
Human IgG (H&L) |
Peroxidase (Horseradish) |
|
|
Goat |
Human IgG F(c) |
Peroxidase (Horseradish) |
|
|
Goat |
Human IgG F(c) |
Alkaline Phosphatase (Calf Intestine) |
|
|
Goat |
Human IgG F(c) |
Biotin |
|
|
Goat |
Human IgG (H&L) |
R-Phycoerythrin (RPE) |
|
|
Goat |
Human IgG F(c) |
R-Phycoerythrin (RPE) |
|
|
Goat |
Human IgG F(c) |
Texas Red® |
|
|
Donkey |
Human IgG (H&L) |
Texas Red® |
|
|
Goat |
Mouse IgG F(c) |
Rhodamine (TRITC) |
|
|
Goat |
Mouse IgG F(c) |
Unconjugated |
|
|
Goat |
Mouse IgG F(ab')2 |
Peroxidase (Horseradish) |
|
|
Goat |
Mouse IgG (H&L) |
Biotin |
|
|
Goat |
Mouse IgG F(c) |
R-Phycoerythrin (RPE) |
|
|
Rabbit |
Mouse IgG (H&L) |
Unconjugated |
|
|
Rabbit |
Mouse IgG (H&L) |
R-Phycoerythrin (RPE) |
|
|
Donkey |
Mouse IgG (H&L) |
Texas Red® |
|
|
Goat |
Rabbit IgG (H&L) |
Texas Red® |
|
|
Goat |
Rabbit IgG (H&L) |
Unconjugated |
|
|
Goat |
Rabbit IgG F(ab')2 |
Unconjugated |
|
|
Goat |
Rabbit IgG F(c) |
Peroxidase (Horseradish) |
|
|
Goat |
Rabbit IgG (H&L) |
Texas Red® |
|
|
Goat |
Rabbit IgG F(ab')2 |
Texas Red® |
|
|
Donkey |
Rabbit IgG (H&L) |
R-Phycoerythrin (RPE) |
|
|
Goat |
Rat IgG F(c) |
Peroxidase (Horseradish) |
|
|
Goat |
Rat IgG (H&L) |
Peroxidase (Horseradish) |
|
|
Donkey |
Goat IgG (H&L) |
Peroxidase (Horseradish) |
|
|
Goat |
Human IgG (H&L) |
Unconjugated |
|
|
Goat |
Human IgG (H&L) |
Peroxidase (Horseradish) |
|
|
Rabbit |
Human IgG (H&L) |
Unconjugated |
|
|
Goat |
Mouse IgG (H&L) |
Unconjugated |
|
|
Goat |
Mouse IgG (H&L) |
Fluorescein (FITC) |
|
|
Goat |
Rabbit IgG (H&L) |
Unconjugated |
|
|
Goat |
Rabbit IgG (H&L) |
Peroxidase (Horseradish) |
|
|
Goat |
Rabbit IgG (H&L) |
Texas Red® |
Selection guide
Step 1. The target species of the secondary antibody need to match the host of the primary antibody
Step 2. The immunogen of the secondary antibody need to match the class (isotype), subclass or the fragment of the primary antibody. Select an appropriate host species for the secondary antibody.
Example:
- Primary antibody: IL-8 Antibody, mAb, Mouse (Cat.no A00044),
- Host: mouse
- Ig Subclass: IgG1
- Secondary antibody: Goat Anti-Mouse IgG Antibody (H&L) [HRP], pAb (Cat.no A00160)
- Host: Goat
- Target species: mouse
- Immunogen: Full length mouse IgG (purified)
- It reacts with All classes, heavy and light chains (H&L) and fragments of mouse IgG.
Immunoglobulin (Ig) Types of Mammals
|
Ig Class (Isotype) |
Subclass |
Heavy Chain |
Light Chain |
Complexes |
|
Ig A |
Ig A1, Ig A2 |
α1, α2 |
κ or λ |
dimer |
|
Ig D |
δ |
monomer |
||
|
Ig E |
ε |
monomer |
||
|
Ig G |
Ig G1, Ig G2a, Ig G2b, Ig G3, Ig G4 |
γ1, γ2, γ3, γ4 |
monomer |
|
|
Ig M |
μ |
pentamer |
Step 3. Choose the label of the secondary antibody according to your application.
|
Application |
Label Type |
Label Options |
|
ELISA |
Enzyme |
Biotin, |
|
Western Blot (WB) |
||
|
Flow Cytometry |
Fluorochrome |
R-Phycoerythrin(RPE), |
|
Immunofluorescence (IF) |
||
|
Immunocytochemistry (ICC) |
||
|
FLISA |
||
|
Immunohistochemistry (IHC) |
Enzyme/Fluorochrome |
Biotinylated secondary antibodies – avidin/streptavidin conjugated to enzyme/fluorochrome |
Step 4. Consider other specificity issues of the secondary antibody