Secondary Antibodies



Secondary Antibodies

A primary antibody's Fc domain is constant within the same animal species, therefore, a given secondary antibody can be used with any primary antibody of the same isotype and target species. This reduces the cost by labeling only one type of secondary antibody. In addition, secondary antibodies offer increased sensitivity through the signal amplification.

At GenScript, secondary antibodies are purified from the serum of several hosts, including goat, donkey, mouse, rabbit, chicken and sheep. Secondary antibodies are available with specificity for whole Ig molecules or antibody fragments such as the Fc or Fab regions. The conjugated secondary antibodies can be used for various immunology analysis and protein assays.

GenScript also offers over 1000 highly specific monoclonal and polyclonal primary antibodies. 

 

<<Featured Products>>

 

MonoRabᵀᴹ Rabbit Anti-Camelid VHH Monoclonal Antibody Series

 

Camelids naturally produce antibodies composed only of heavy chains as compared to the conventional antibodies (Fig 1)The antigen-binding site of these unusual heavy chain antibodies is formed only by a single heavy chain variable domain, designated as single domain antibodies (sdAbs or VHHs). Because of their unique structure, small size and stability, VHHs have several advantages comparing to conventional antibodies or their fragments. Several VHHs are now being studied for use in the diagnostic fields and therapeutic areas, including oncology, infectious, inflammatory, and neurodegenerative diseases.

 

Rabbit anti-camelid VHH monoclonal antibody series generated by Genscript's proprietary rabbit-mouse hetero-hybridoma technology MonoRab™ provide perfect secondary antibody tools for researchers to identify target-specific sdAbs.

 

Cat. No.

Product Name

A01860-200

MonoRabᵀᴹ Rabbit Anti-Camelid VHH Antibody, mAb - 200 µl

A01995-200

MonoRabᵀᴹ Rabbit Anti-Camelid VHH Antibody [Biotin], mAb - 200 µl

A01861-200

MonoRabᵀᴹ Rabbit Anti-Camelid VHH Antibody [HRP], mAb - 200 µl

A01862-200

MonoRabᵀᴹ Rabbit Anti-Camelid VHH Antibody [iFluor488], mAb - 200 µl

A01863-200

MonoRabᵀᴹ Rabbit Anti-Camelid VHH Antibody [iFluor555], mAb - 200 µl

A01994-200

MonoRabᵀᴹ Rabbit Anti-Camelid VHH Antibody [iFluor 647], mAb-200µl


 


Key Features

  • MonoRab™ technology guarantees high affinity: Kd 10-11 M
  • No cross reactivity with other species
  • Recognizes the variable domain of camelid (Llama and Camel) antibodies
  • Specific to camelid IgG2 & IgG3
  • Recognizes conformational epitope

 

 


Mouse Anti-Rabbit IgG Antibody (M205) [HRP], mAb

 

Conventional polyclonal antibodies recognize many epitopes, leading to high background and cross reactivity in many assays. M205 is specific to native rabbit IgG and doesn't react with denatured or reduced rabbit IgG heavy or light chain. It's an ideal tool for Immunoprecipitation and Western blot. Primary antibody heavy and light chain contamination can be avoided.


 


The result demonstrates that Anti-Rabbit IgG Monoclonal Secondary Antibody (M205) provides less background noise than conventional polyclonal secondary antibody with equivalent sensitivity.

 

 


Detection of different titrated primary rabbit antibodies using Anti-Rabbit IgG Monoclonal Secondary Antibody (M205) (HRP conjugate)#A1827 indicates that M205 binds to a conserved rabbit IgG epitope, and can recognize primary antibodies generated from different rabbits.

 

All Products

 

A00098

Goat

Rabbit IgG (H&L)

Peroxidase (Horseradish)

A00131

Goat

Rabbit IgG (H&L)

Unconjugated

A00160

Goat

Mouse IgG (H&L)

Peroxidase (Horseradish)

A00165

Goat

Chicken IgY (H&L)

Peroxidase (Horseradish)

A00166

Goat

Human IgG (H&L)

Peroxidase (Horseradish)

A00167

Goat

Rat IgG (H&L)

Peroxidase (Horseradish)

A00178

Donkey

Goat IgG

Peroxidase (Horseradish)

A01318

Goat

Mouse IgG (H&L)

Fluorescein (FITC)

A01827-200

Mouse

Rabbit IgG

Peroxidase (Horseradish)

A01854-200

Mouse

Human IgG F(c)

Peroxidase (Horseradish)

A01855-200

Mouse

Human IgG F(ab)

Peroxidase (Horseradish)

A01856-200

Mouse

Rabbit IgG F(c)

Peroxidase (Horseradish)

A01860-200

Rabbit

Camelid VHH

Unconjugated

A01861-200

Rabbit

Camelid VHH

Peroxidase (Horseradish)

A01862-200

Rabbit

Camelid VHH

iFluor488

A01863-200

Rabbit

Camelid VHH

iFluor555

A10001

Goat

Mouse IgM (H&L)

Unconjugated

A10002

Rabbit

Mouse IgM (H&L)

Unconjugated

A10003

Goat

Mouse IgM (H&L)

Fluorescein (FITC)

A10005

Rabbit

Mouse IgM (H&L)

Unconjugated

A10006

Rabbit

Mouse IgM (H&L)

Fluorescein (FITC)

A10007

Rabbit

Mouse IgM (H&L)

Peroxidase (Horseradish)

A10008

Rabbit

Bovine IgG F(ab')2

Texas Red®

A10009

Goat

Chicken IgG (H&L)

Texas Red®

A10010

Rabbit

Chicken IgG (H&L)

Texas Red®

A10011

Goat

Dog IgG F(c)

Texas Red®

A10013

Rabbit

Goat IgG F(c)

Peroxidase (Horseradish)

A10014

Rabbit

Goat IgG (H&L)

Texas Red®

A10015

Donkey

Goat IgG (H&L)

Peroxidase (Horseradish)

A10016

Donkey

Goat IgG (H&L)

Alkaline Phosphatase (Calf Intestine)

A10017

Donkey

Goat IgG (H&L)

Biotin

A10018

Donkey

Goat IgG (H&L)

Texas Red®

A10019

Goat

Guinea Pig IgG (H&L)

Texas Red®

A10020

Goat

Human IgG F(c)

Unconjugated

A10021

Goat

Human IgG (H&L)

Unconjugated

A10022

Goat

Human IgG (H&L)

Fluorescein (FITC)

A10023

Goat

Human IgG F(c)

Peroxidase (Horseradish)

A10024

Goat

Human IgG (H&L)

Peroxidase (Horseradish)

A10025

Goat

Human IgG IgA IgM

Peroxidase (Horseradish)

A10026

Goat

Human IgG (H&L)

Alkaline Phosphatase (Calf Intestine)

A10027

Goat

Human IgG IgA IgM

Alkaline Phosphatase (Calf Intestine)

A10028

Goat

Human IgG F(c)

Biotin

A10029

Goat

Human IgG (H&L)

Biotin

A10030

Goat

Human IgG (H&L)

R-Phycoerythrin (RPE)

A10031

Goat

Human IgG (H&L)

Unconjugated

A10032

Goat

Human IgG F(c)

Unconjugated

A10033

Goat

Human IgG F(ab')2

Unconjugated

A10034

Goat

Human IgM µ chain

Unconjugated

A10035

Goat

Human κ chain

Unconjugated

A10036

Goat

Human λ chain

Unconjugated

A10037

Goat

Human IgG (H&L)

Unconjugated

A10038

Goat

Human IgG (H&L)

Fluorescein (FITC)

A10039

Goat

Human IgG (H&L)

Peroxidase (Horseradish)

A10040

Goat

Human IgG F(c)

Peroxidase (Horseradish)

A10041

Goat

Human IgG F(ab')2

Peroxidase (Horseradish)

A10042

Goat

Human IgM µ chain

Peroxidase (Horseradish)

A10043

Goat

Human IgG (H&L)

Peroxidase (Horseradish)

A10044

Goat

Human IgG F(ab')2

Biotin

A10045

Goat

Human κ chain

Biotin

A10047

Goat

Human IgG (H&L)

Texas Red®

A10048

Mouse

Human IgG (H&L)

Unconjugated

A10049

Rabbit

Human IgG (H&L)

Unconjugated

A10050

Rabbit

Human IgA

Unconjugated

A10051

Rabbit

Human IgG F(ab')2

Unconjugated

A10053

Rabbit

Human IgG (H&L)

Peroxidase (Horseradish)

A10054

Rabbit

Human IgG F(c)

Peroxidase (Horseradish)

A10055

Rabbit

Human IgG (γ chain)

Peroxidase (Horseradish)

A10056

Rabbit

Human IgG (H&L)

Peroxidase (Horseradish)

A10057

Rabbit

Human IgG F(ab')2

Peroxidase (Horseradish)

A10058

Rabbit

Human IgM Fc5µ

Peroxidase (Horseradish)

A10059

Rabbit

Human IgG (H&L)

Alkaline Phosphatase (Calf Intestine)

A10060

Rabbit

Human IgG F(c)

Alkaline Phosphatase (Calf Intestine)

A10061

Rabbit

Human IgG F(c)

Biotin

A10062

Rabbit

Human IgA

Biotin

A10063

Rabbit

Human IgG (H&L)

Texas Red®

A10064

Rabbit

Human IgG F(c)

Texas Red®

A10065

Rabbit

Human IgA

Texas Red®

A10066

Sheep

Human IgG (H&L)

Peroxidase (Horseradish)

A10067

Sheep

Human IgG (H&L)

Biotin

A10068

Chicken

Human IgG (H&L)

Peroxidase (Horseradish)

A10069

Chicken

Human IgG (H&L)

Texas Red®

A10070

Goat

Mouse IgG + IgM (H&L)

Rhodamine (TRITC)

A10071

Goat

Mouse IgG (H&L)

Rhodamine (TRITC)

A10072

Goat

Mouse IgG (H&L)

Rhodamine (TRITC)

A10073

Goat

Mouse IgG (H&L)

Unconjugated

A10074

Goat

Mouse IgG IgA IgM

Unconjugated

A10075

Goat

Mouse IgG + IgM (H&L)

Fluorescein (FITC)

A10076

Goat

Mouse IgG (H&L)

Fluorescein (FITC)

A10077

Goat

Mouse IgG1

Peroxidase (Horseradish)

A10078

Goat

Mouse IgG2b

Peroxidase (Horseradish)

A10079

Goat

Mouse IgG + IgM (H&L)

Peroxidase (Horseradish)

A10080

Goat

Mouse IgG (H&L)

Peroxidase (Horseradish)

A10081

Goat

Mouse IgG IgA IgM

Peroxidase (Horseradish)

A10082

Goat

Mouse IgG IgA IgM

Peroxidase (Horseradish)

A10083

Goat

Mouse IgG (H&L)

Alkaline Phosphatase (Calf Intestine)

A10084

Goat

Mouse IgG (H&L)

Biotin

A10085

Goat

Mouse IgG (H&L)

R-Phycoerythrin (RPE)

A10086

Goat

Mouse IgG (H&L)

Texas Red®

A10087

Goat

Mouse IgG (H&L)

Unconjugated

A10088

Goat

Mouse IgG F(c)

Unconjugated

A10089

Goat

Mouse IgM µ chain

Unconjugated

A10090

Goat

Mouse IgG (H&L)

Unconjugated

A10091

Goat

Mouse IgG (H&L)

Fluorescein (FITC)

A10092

Goat

Mouse IgG F(ab')2

Fluorescein (FITC)

A10093

Goat

Mouse IgG (H&L)

Peroxidase (Horseradish)

A10094

Goat

Mouse IgG F(c)

Peroxidase (Horseradish)

A10095

Goat

Mouse IgG F(ab')2

Peroxidase (Horseradish)

A10096

Goat

Mouse IgG (H&L)

Peroxidase (Horseradish)

A10097

Goat

Mouse IgG (H&L)

Alkaline Phosphatase (Calf Intestine)

A10098

Goat

Mouse IgG (H&L)

Alkaline Phosphatase (Calf Intestine)

A10099

Goat

Mouse IgG (H&L)

Biotin

A10100

Goat

Mouse IgG (H&L)

Texas Red®

A10101

Rabbit

Mouse IgG (H&L)

Rhodamine (TRITC)

A10102

Rabbit

Mouse κ chain

Rhodamine (TRITC)

A10104

Rabbit

Mouse IgG (H&L)

Unconjugated

A10105

Rabbit

Mouse IgG F(c)

Unconjugated

A10106

Rabbit

Mouse IgM µ chain

Unconjugated

A10107

Rabbit

Mouse κ chain

Unconjugated

A10108

Rabbit

Mouse λ chain

Unconjugated

A10109

Rabbit

Mouse IgG (γ chain)

Unconjugated

A10110

Rabbit

Mouse IgG (H&L)

Unconjugated

A10113

Rabbit

Mouse IgG2b

Unconjugated

A10114

Rabbit

Mouse IgG3

Unconjugated

A10115

Rabbit

Mouse IgG (H&L)

Fluorescein (FITC)

A10116

Rabbit

Mouse IgG1

Fluorescein (FITC)

A10117

Rabbit

Mouse IgG2a

Fluorescein (FITC)

A10119

Rabbit

Mouse IgG F(c)

Peroxidase (Horseradish)

A10120

Rabbit

Mouse κ chain

Peroxidase (Horseradish)

A10121

Rabbit

Mouse IgG (γ chain)

Peroxidase (Horseradish)

A10122

Rabbit

Mouse IgG (H&L)

Peroxidase (Horseradish)

A10125

Rabbit

Mouse IgG2b

Peroxidase (Horseradish)

A10126

Rabbit

Mouse IgG3

Peroxidase (Horseradish)

A10127

Rabbit

Mouse IgG (H&L)

Alkaline Phosphatase (Calf Intestine)

A10128

Rabbit

Mouse IgA

Alkaline Phosphatase (Calf Intestine)

A10129

Rabbit

Mouse κ chain

Alkaline Phosphatase (Calf Intestine)

A10130

Rabbit

Mouse λ chain

Alkaline Phosphatase (Calf Intestine)

A10131

Rabbit

Mouse IgG (H&L)

Alkaline Phosphatase (Calf Intestine)

A10132

Rabbit

Mouse IgG1

Alkaline Phosphatase (Calf Intestine)

A10133

Rabbit

Mouse IgG2a

Alkaline Phosphatase (Calf Intestine)

A10134

Rabbit

Mouse IgG (H&L)

Biotin

A10135

Rabbit

Mouse IgG F(c)

Biotin

A10136

Rabbit

Mouse IgM µ chain

Biotin

A10137

Rabbit

Mouse IgG1

Biotin

A10138

Rabbit

Mouse IgG2a

Biotin

A10139

Rabbit

Mouse IgG F(c)

Texas Red®

A10140

Rabbit

Mouse IgA

Texas Red®

A10141

Rabbit

Mouse IgG (γ chain)

Texas Red®

A10143

Sheep

Mouse IgG (H&L)

Fluorescein (FITC)

A10145

Sheep

Mouse IgG (H&L)

Texas Red®

A10146

Donkey

Mouse IgG (H&L)

Unconjugated

A10147

Donkey

Mouse IgG (H&L)

Fluorescein (FITC)

A10149

Donkey

Mouse IgG (H&L)

Peroxidase (Horseradish)

A10150

Donkey

Mouse IgG (H&L)

Biotin

A10151

Donkey

Mouse IgG (H&L)

Texas Red®

A10152

Donkey

Mouse IgG (H&L)

Texas Red®

A10153

Chicken

Mouse IgG (H&L)

Unconjugated

A10154

Chicken

Mouse IgG (H&L)

Peroxidase (Horseradish)

A10155

Goat

Rabbit IgG (H&L)

Rhodamine (TRITC)

A10157

Goat

Rabbit IgG (H&L)

Unconjugated

A10158

Goat

Rabbit IgG (H&L)

Fluorescein (FITC)

A10159

Goat

Rabbit IgG F(c)

Rhodamine (TRITC)

A10160

Goat

Rabbit IgG (H&L)

Peroxidase (Horseradish)

A10161

Goat

Rabbit IgG (H&L)

Alkaline Phosphatase (Calf Intestine)

A10162

Goat

Rabbit IgG (H&L)

Biotin

A10163

Goat

Rabbit IgG (H&L)

Unconjugated

A10164

Goat

Rabbit IgG F(c)

Unconjugated

A10165

Goat

Rabbit IgG (H&L)

Unconjugated

A10166

Goat

Rabbit IgG F(c)

Unconjugated

A10167

Goat

Rabbit IgG (H&L)

Fluorescein (FITC)

A10168

Goat

Rabbit IgG (H&L)

Fluorescein (FITC)

A10169

Goat

Rabbit IgG (H&L)

Peroxidase (Horseradish)

A10170

Goat

Rabbit IgG F(c)

Peroxidase (Horseradish)

A10171

Goat

Rabbit IgG F(ab')2

Peroxidase (Horseradish)

A10172

Goat

Rabbit IgG (H&L)

Peroxidase (Horseradish)

A10173

Goat

Rabbit IgG F(c)

Peroxidase (Horseradish)

A10174

Goat

Rabbit IgG (H&L)

Alkaline Phosphatase (Calf Intestine)

A10175

Goat

Rabbit IgG F(c)

Alkaline Phosphatase (Calf Intestine)

A10176

Goat

Rabbit IgG (H&L)

Alkaline Phosphatase (Calf Intestine)

A10177

Goat

Rabbit IgG F(c)

Alkaline Phosphatase (Calf Intestine)

A10178

Goat

Rabbit IgG (H&L)

Biotin

A10179

Goat

Rabbit IgG F(c)

Biotin

A10180

Goat

Rabbit IgG F(c)

Biotin

A10181

Goat

Rabbit IgG (H&L)

Texas Red®

A10182

Goat

Rabbit IgG F(c)

Texas Red®

A10183

Goat

Rabbit IgG F(c)

Texas Red®

A10184

Mouse

Rabbit IgG (H&L)

Unconjugated

A10185

Sheep

Rabbit IgG (H&L)

Peroxidase (Horseradish)

A10186

Donkey

Rabbit IgG (H&L)

Rhodamine (TRITC)

A10187

Donkey

Rabbit IgG F(c)

Unconjugated

A10188

Donkey

Rabbit IgG (H&L)

Unconjugated

A10189

Donkey

Rabbit IgG F(c)

Fluorescein (FITC)

A10190

Donkey

Rabbit IgG (H&L)

Fluorescein (FITC)

A10191

Donkey

Rabbit IgG F(c)

Peroxidase (Horseradish)

A10192

Donkey

Rabbit IgG (H&L)

Peroxidase (Horseradish)

A10193

Donkey

Rabbit IgG (H&L)

Alkaline Phosphatase (Calf Intestine)

A10194

Donkey

Rabbit IgG F(c)

Biotin

A10195

Donkey

Rabbit IgG (H&L)

Biotin

A10196

Donkey

Rabbit IgG (H&L)

Texas Red®

A10197

Donkey

Rabbit IgG (H&L)

Fluorescein (FITC)

A10198

Donkey

Rabbit IgG (H&L)

Peroxidase (Horseradish)

A10199

Donkey

Rabbit IgG (H&L)

Alkaline Phosphatase (Calf Intestine)

A10200

Donkey

Rabbit IgG (H&L)

Biotin

A10201

Donkey

Rabbit IgG (H&L)

Texas Red®

A10202

Chicken

Rabbit IgG (H&L)

Peroxidase (Horseradish)

A10203

Goat

Rat IgG (H&L)

Unconjugated

A10204

Goat

Rat IgG (H&L)

Peroxidase (Horseradish)

A10205

Goat

Rat IgG (H&L)

Biotin

A10206

Goat

Rat IgG (H&L)

R-Phycoerythrin (RPE)

A10207

Goat

Rat IgG F(c)

Unconjugated

A10208

Goat

Rat IgM µ chain

Unconjugated

A10209

Goat

Rat IgG (H&L)

Fluorescein (FITC)

A10210

Goat

Rat IgG F(c)

Fluorescein (FITC)

A10211

Goat

Rat IgG (H&L)

Peroxidase (Horseradish)

A10212

Goat

Rat IgG F(c)

Peroxidase (Horseradish)

A10214

Goat

Rat IgM µ chain

Peroxidase (Horseradish)

A10215

Goat

Rat IgG (H&L)

Alkaline Phosphatase (Calf Intestine)

A10216

Goat

Rat IgG (H&L)

Biotin

A10217

Goat

Rat IgG F(c)

Biotin

A10218

Rabbit

Rat IgG (H&L)

Unconjugated

A10219

Rabbit

Rat IgG (H&L)

Fluorescein (FITC)

A10220

Rabbit

Rat IgG (H&L)

Peroxidase (Horseradish)

A10221

Rabbit

Rat IgG (H&L)

Biotin

A10222

Rabbit

Rat IgG (H&L)

Texas Red®

A10223

Sheep

Rat IgG (H&L)

Unconjugated

A10224

Donkey

Rat IgG (H&L)

Unconjugated

A10225

Donkey

Rat IgG (H&L)

Peroxidase (Horseradish)

A10226

Donkey

Rat IgG (H&L)

Texas Red®

A10227

Rabbit

Sheep IgG (H&L)

Texas Red®

A10228

Goat

Swine IgG (H&L)

Texas Red®

A10229

Goat

Monkey IgA

Unconjugated

A10230

Goat

Monkey IgG (γ chain)

Unconjugated

A10231

Goat

Monkey IgG IgA IgM

Unconjugated

A10232

Goat

Monkey IgA

Fluorescein (FITC)

A10233

Goat

Monkey IgG (γ chain)

Fluorescein (FITC)

A10234

Goat

Monkey IgA

Peroxidase (Horseradish)

A10235

Goat

Monkey IgM µ chain

Peroxidase (Horseradish)

A10236

Goat

Monkey IgG (γ chain)

Peroxidase (Horseradish)

A10238

Goat

Monkey IgA

Biotin

A10239

Goat

Monkey IgG (γ chain)

Biotin

A10240

Goat

Armenian Hamster IgG (H&L)

Peroxidase (Horseradish)

A10241

Donkey

Goat IgG (H&L)

Peroxidase (Horseradish)

A10242

Donkey

Goat IgG (H&L)

R-Phycoerythrin (RPE)

A10243

Goat

Human IgG IgA IgM

Unconjugated

A10244

Goat

Human IgG IgA IgM

Peroxidase (Horseradish)

A10245

Goat

Human IgG IgA IgM

Alkaline Phosphatase (Calf Intestine)

A10246

Goat

Human IgA

Unconjugated

A10247

Goat

Human κ chain

Unconjugated

A10248

Goat

Human IgG (H&L)

Unconjugated

A10249

Goat

Human IgG F(c)

Unconjugated

A10250

Goat

Human κ chain

Fluorescein (FITC)

A10251

Goat

Human λ chain

Fluorescein (FITC)

A10252

Goat

Human IgA

Peroxidase (Horseradish)

A10253

Goat

Human IgG (H&L)

Peroxidase (Horseradish)

A10254

Goat

Human IgG F(c)

Peroxidase (Horseradish)

A10255

Goat

Human IgG F(c)

Alkaline Phosphatase (Calf Intestine)

A10256

Goat

Human IgG F(c)

Biotin

A10257

Goat

Human IgG (H&L)

R-Phycoerythrin (RPE)

A10258

Goat

Human IgG F(c)

R-Phycoerythrin (RPE)

A10259

Goat

Human IgG F(c)

Texas Red®

A10260

Donkey

Human IgG (H&L)

Texas Red®

A10261

Goat

Mouse IgG F(c)

Rhodamine (TRITC)

A10262

Goat

Mouse IgG F(c)

Unconjugated

A10263

Goat

Mouse IgG F(ab')2

Peroxidase (Horseradish)

A10265

Goat

Mouse IgG (H&L)

Biotin

A10266

Goat

Mouse IgG F(c)

R-Phycoerythrin (RPE)

A10267

Rabbit

Mouse IgG (H&L)

Unconjugated

A10268

Rabbit

Mouse IgG (H&L)

R-Phycoerythrin (RPE)

A10269

Donkey

Mouse IgG (H&L)

Texas Red®

A10270

Goat

Rabbit IgG (H&L)

Texas Red®

A10271

Goat

Rabbit IgG (H&L)

Unconjugated

A10272

Goat

Rabbit IgG F(ab')2

Unconjugated

A10273

Goat

Rabbit IgG F(c)

Peroxidase (Horseradish)

A10274

Goat

Rabbit IgG (H&L)

Texas Red®

A10275

Goat

Rabbit IgG F(ab')2

Texas Red®

A10276

Donkey

Rabbit IgG (H&L)

R-Phycoerythrin (RPE)

A10277

Goat

Rat IgG F(c)

Peroxidase (Horseradish)

A10278

Goat

Rat IgG (H&L)

Peroxidase (Horseradish)

A10279

Donkey

Goat IgG (H&L)

Peroxidase (Horseradish)

A10280

Goat

Human IgG (H&L)

Unconjugated

A10281

Goat

Human IgG (H&L)

Peroxidase (Horseradish)

A10282

Rabbit

Human IgG (H&L)

Unconjugated

A10283

Goat

Mouse IgG (H&L)

Unconjugated

A10284

Goat

Mouse IgG (H&L)

Fluorescein (FITC)

A10286

Goat

Rabbit IgG (H&L)

Unconjugated

A10287

Goat

Rabbit IgG (H&L)

Peroxidase (Horseradish)

A10288

Goat

Rabbit IgG (H&L)

Texas Red®

 


Selection guide

 

Step 1. The target species of the secondary antibody need to match the host of the primary antibody

 

Step 2. The immunogen of the secondary antibody need to match the class (isotype), subclass or the fragment of the primary antibody. Select an appropriate host species for the secondary antibody.

 

Example:

 

Immunoglobulin (Ig) Types of Mammals

Ig Class (Isotype)

Subclass

Heavy Chain

Light Chain

Complexes

Ig A

Ig A1, Ig A2

α1, α2

κ or λ

dimer

Ig D

δ

monomer

Ig E

ε

monomer

Ig G

Ig G1, Ig G2a, Ig G2b, Ig G3, Ig G4

γ1, γ2, γ3, γ4

monomer

Ig M

μ

pentamer

 

Step 3. Choose the label of the secondary antibody according to your application.

 

Application

Label Type

Label Options

ELISA

Enzyme

Biotin, 
Alkaline Phosphatase (AP), 
Horseradish Peroxidase (HRP)

Western Blot (WB)

Flow Cytometry

Fluorochrome

R-Phycoerythrin(RPE), 
Fluorescein (FITC), 
Texas Red ® , 
Rhodamine (TRITC)

Immunofluorescence (IF)

Immunocytochemistry (ICC)

FLISA

Immunohistochemistry (IHC)

Enzyme/Fluorochrome

Biotinylated secondary antibodies – avidin/streptavidin conjugated to enzyme/fluorochrome

 

Step 4. Consider other specificity issues of the secondary antibody

 

  • Antibody fragment

If working with tissues or cells that have Fc receptors (spleen, blood, hematopoietic cells, leukocytes, etc.), choose a F(ab) or F(ab')2 fragment to eliminate non-specific binding.  
F(ab')2 fragment secondary antibodies are generated by pepsin digestion of whole IgG antibodies to remove most of the Fc region while leaving intact the hinge region. Two F(ab) fragments and an Fc fragment are generated if digested by papain.

 

  • Pre-adsorbed (also cross-adsorbed)

The pre-adsorption process reduces the risk of cross reactivity between the secondary antibody and endogenous immunoglobulins present on cell and tissue samples. The process involves passing the secondary antibody over immobilized serum proteins from potentiAlly cross reactive species. 
Example: Goat Anti-Mouse IgG (H&L) [TRITC], pAb (Cat.no A10071) is pre-adsorbed and has Min X Bv Ch Gt GP Ham Hs Hu Rb Rt & Sh Serum Proteins.