Consumable



qPCRBIO cDNA synthesis kit
상세설명
제품용도 cDNA synthesis
브랜드 PCR Biosystems
제품개요 단 2개의 tube로 간편하게 cDNA 합성이 가능한 제품
제품소개
qPCRBIO cDNA synthesis kit는 RNA에서 높은 수율의 cDNA 합성이 가능한 제품이며 열에 안정한 변형 된 M-MLV Reverse Transcription을 기반으로 개발된 제품이기 때문에 온도에 안정적이고, 최상의 활성을 유지 할 수 있습니다. 이 제품은 효율적인 cDNA 합성을 위해 최적의 buffer와 random hexamer, oligo(dT)를 모두 포함하여 5x mix 형태로 제공 됩니다.
제품특징
• Unbiased representation of 5’ and 3’ mRNA transcript ends
• 최소 1pg부터 4ug의 RNA 사용 가능
• 간단한 2-tube system
• 30분 protocol

응용분야
• cDNA synthesis for real-time PCR analysis
• Low copy number transcripts
• Viral RNA targets / miRNA targets
• Efficient synthesis from total RNA or poly(A)+ RNA

보관온도
 
• -20℃

 

Figure 1. Broad reverse transcription dynamic range. A 10 fold serial dilution of mouse total RNA was used for 7 cDNA synthesis reactions. The highest amount of RNA used for cDNA synthesis was 4μg and the lowest 4pg. Each cDNA synthesis reaction was used as a template for a qPCRBIO SyGreen reation, amplifying a 70bp fragment of the mouse RN18S gene. Reactions were incubated for 30minutes at 42˚C. Efficiency was measured at 100% across the range tested. The results demonstrate that qPCRBIO cDNA Synthesis Kit efficiently reverse transcribes RNA across a broad dynamic range of substrate. 




 

   

 
Figure 2a. Unbiased representation of mRNA ends. qPCRBIO cDNA synthesis kit was used to synthesis cDNA from mouse liver total RNA. 2 Primer pairs were designed against the 5’ (red traces) and the 3’ (black traces) ends of the 4.2kb mouse cANX transcript. qPCRBIO SyGreen Mix was used for analysis. The primer pairs were 4kb apart and did not show any reverse transcription bias, hence the amplification traces overlap. 

Figure 2b. Unbiased representation of mRNA ends. qPCRBIO cDNA synthesis kit was used to synthesis cDNA from mouse liver total RNA. 2 primer pairs against the 5’ (red) and 3’ (black) traces of RNS18
 
gene (1.8kb). Again, no reverse transcription bias was evident.
 


 

 

주문정보 

  

  
Product Name

Pack Size          

Cat No.          

Presentation          

qPCRBIO  

cDNA Synthesis Kit          

25 rxns          

PB30.11-02          

[1 x 0.1ml] & [1 x 0.25ml]          

100 rxns          

PB30.11-10          

[4 x 0.1ml] & [1 x 0.1ml]

 

Product Name

Pack Size          

Cat No.          

Presentation          

UltraScript 2.0 

cDNA Synthesis Kit          

25 rxns          

PB30.31-02          

[1 x 25μL UltraScript 2.0] & 

[1 x 100μL reaction mix]            

100 rxns          

PB30.31-10          

[1 x 100μL UltraScript 2.0] & 

[4 x 100μL reaction mix]

 

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