Consumable



PCRBIO UltraScript Reverse Transcriptase
상세설명
제품용도 Reverse Transcriptase
브랜드 PCR Biosystems
제품개요 낮은 RNA 농도로부터 고수율의 cDNA를 합성하는 reverse transcripase
제품소개
UltraScript Reverse Transcriptase는 기존 M-MLV보다 비교적 열에 안정성이 있고 최적의 활성을 갖도록 고안 되었습니다. 저온에서 2차 구조를 형성하는 RNA의 특징을 억제하기 위해 thermostable한 enzyme의 사용으로 온도를 최대 55℃까지 증가시킬 수 있어 cDNA의 합성효율을 증가 시켰습니다. 본 제품은 RTase와 5x buffer로 구성되어 있으며, 5x buffer안에 dNTPs, MgCl2 가 혼합 되어 있어 편리하게 사용할 수 있습니다.
제품특징
• Thermostable reverse transcriptase 45℃ - 55℃
• Advanced RNase Inhibitor
• 최소 4pg의 total RNA로부터 높은 cDNA 수율

응용분야
• Random hexamer, oligo-dT, gene-specific primers
• cDNA synthesis for PCR analysis, cloning, library preparation and Next Generation Sequencing
• Low copy number transcripts
• Viral RNA targets / miRNA targets
• Efficient synthesis from total RNA or poly(A)+ RNA

보관온도
• -20℃
• Stable for 4 weeks at 4℃

  

Figure 1. Thermostable enzyme up to 55°C. The thermostability of UltraScript Reverse Transcriptase was measured using the Sypro Orange fluorescence assay. The protein is incubated with Sypro Orange dye and the temperature gradually increased. The fluorescence intensity increases as the protein unfolds and the melting point is the temperature where 50% of the protein is unfolded. The DSF curve shows UltraScript Reverse Transcriptase (purple) and wildtype MMLV RT (black) at 0.1mg/ml. This experiment shows that UltraScript Reverse Transcriptase unfolds at 54.9±0.3°C, which is 9.3°C higher than the wildtype enzyme, indicating it is more thermostable and more likely to remain active during the reaction.
 


Figure 2. Generation of high cDNA yields DNA was created from 100ng of total RNA from mouse liver using UltraScript Reverse Transcriptase (purple) and a competitor mix (blue The reaction was incubated for 10 minutes at 42°C. The resulting cDNA was quantified using qPCRBIO SyGreen Mix. PCR Biosystems UltraScript Reverse Transcriptase created >10x more cDNA than the competitor in the same amount of time. 




 

주문정보   
Product Name

Pack Size

Cat No.

Presentation

UltraScript Reverse transcriptase

10,000 units 

PB30.12-01

[2 x 25ul 200 units/μl] & [1 x 200ul buffer]

40,000 units 

PB30.12-04

[2 x 100ul 200 units/μl] & [4 x 200ul buffer]

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