Consumable



qPCRBIO SyGreen Blue Mix
상세설명
제품용도 Real-Time PCR Reagent
브랜드 PCR Biosystems
제품개요 Blue dye가 포함되어 있어 눈으로 확인하며 Real-time PCR에서 SYBR assay에 사용
제품소개
이 제품은 최신의 buffer와 진보된 hot-start DNA polymerase를 기반으로 최상의 민감도와 재현성을 제공합니다.
qPCRBIO SyGreen Blue Mix는 hot start polymerase, dNTPs, buffer, MgCl2, Intercalating dye를 모두 혼합해 놓은 형태로 primer, template만 넣고 PCR을 수행할 수 있도록 하였습니다. 다양한 기종에 맞춰 ROX reference dye를 혼합한 제품으로 보다 손쉽게 사용할 수 있도록 하였으며 PCR에 영향을 주지 않는 비반응성 Blue dye를 첨가 함으로써 reaction을 하는 동안에 sample을 눈으로 확인 할 수 있습니다.
제품특징
• Fast and Sensitivity
• Easy sample visualisation

응용분야
• Absolute quantification
• Relative gene expression analysis
• Low copy number target genes

보관온도
• -20℃
• Stable for 6 months at 25℃ - 37℃


Figure. 1 qPCRBIO SyGreen Blue Mix for  greater pipetting precision.

qPCRBIO SyGreen Blue Mix contains a non-reactive blue dye for easy

sample visualisation and greater precision, particularly useful when

loading small volume reactions. 

 


 

 

  


 

Figure 2. Amplification and melt traces of 4 mouse housekeeping genes from a cDNA dilution series. 

ACTG1: qPCRBIO mix was 2-4 Ct values earlier than 3 competitor mixes and equal to K. The sensitivity was equal to 3 mixes but superior to K, demonstrated by the absence of primer dimer at low template concentrations. GAPDH: qPCRBIO mix was 1-3 Ct values earlier than 2 competitor mixes and equal to 2 mixes. The sensitivity was superior to 3 competitor mixes, demonstrated by the absence of primer dimer, and equal to A. PGK: qPCRBIO mix had Ct values equal or lower than the 4 competitor mixes. Sensitivity was superior to 2 mixes and equal to 2 mixes. CANX: Ct values were 1-6 lower than 3 of 4 mixes and equal to K. Sensitivity was equal to 2 mixes and superior to 2 mixes. Overall qPCRBIO SyGreen Mix outperforms each competitor mix on the 4 amplicons tested.

주문정보    
Product Name

Pack Size

Cat No.

Presentation    

Compatible Model

qPCRBIO SyGreen Blue Mix Hi-ROX

500 rxns

PB20.16-05

5 x 1ml

ABI 7000,7300,7700,7900,7900HT StepOnePlusTM; StepOneTM; OpenArray PRISMTM Seqencing Detecion Series

2000 rxns

PB20.16-20

     

      20 x 1ml                 

qPCRBIO SyGreen Blue Mix Lo-ROX

500 rxns

PB20.15-05

5 x 1ml

ABI 7500, ViiaTM; QuantStudio; IIumina Eco; Stratagene Mx3000, MX3005, Mx4000

2000 rxns

PB20.15-20

20 x 1ml

qPCRBIO SyGreen Blue Mix Separate-Rox

500 rxns

PB20.17-05

5 x 1ml

BioRad CFX96, CFX384, Chromo4TM, CFXconnectTM, MiniOpticonTM; Roche LightCylcer (2.0,1.5,480,1536, Nano); MJ Research OpticonTM OpticonTM2, Chroma 4; Corbett Rotor-Gene (3000,6200,62H0,6500,65H0,6600)

2000 rxns

PB20.17-20

20 x 1ml

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