Consumable



qPCRBIO Probe Mix
상세설명
제품용도 Real Time PCR Reagent
브랜드 PCR Biosystems
제품개요 Real-time PCR에서 Dual-labeled Probe 실험에 사용
제품소개
qPCRBIO Probe Mix는 real-time PCR에서 일반적으로 사용되고 있는 모든 probe를 기반으로 개발 되었습니다. Hot start polymerase, dNTPs, buffer, MgCl2를 모두 혼합해 놓은 형태로 primer, sequence specific probe, template만 넣고 PCR을 수행할 수 있도록 하였습니다. 다양한 기종에 맞춰 ROX reference dye를 혼합한 제품으로 보다 손쉽게 사용할 수 있도록 하였습니다.
제품특징
• Fast and Sensitivity
• High efficiency in multiplex reactions
• Easy sample visualization

응용분야
• Absolute quantification
• Relative gene expression analysis
• Low copy number target genes
• TaqMan®, Scorpions® and molecular beacon probes
• Multiplex or singleplex
• Diagnostic real-time PCR
    
보관온도
• -20℃          
• Stable for 6 months at 25℃ - 37℃


 Figure2. Experiments 1-3 show TaqMan® probe amplification traces of human gene ACVR2B in singleplex and in quadplex (ACVR2B, LIMK1, ACVR1B and CDK7) from a cDNA dilution series. qPCRBIO Probe Mix shows the least PCR inhibition when in multiplex compared to I and B mixes. This is evident in more delayed amplification traces in quadplex (b) compared to singleplex (a). Experiments 4-6 show TaqMan® probe amplification traces of human gene LIMK1 in singleplex and quadplex (ACVR2B, LIMK1, ACVR1B and CDK7). As with experiments 1-3, LIMK1 amplification is less inhibited in multiplex in the qPCRBIO Probe Mix than the competitor mixes tested. 
주문정보    
Product Name

Pack Size

Cat No.

Presentation

Compatible model

qPCRBIO Probe Mix Hi-ROX 

500 

rxns

PB20.22-05

 

5 x 1ml

ABI 7000,7300,7700,7900,7900HT StepOnePlusTM; StepOneTM; OpenArray PRISMTM Seqencing Detecion Series

2000 rxns

PB20.22-20

20 x 1ml

qPCRBIO Probe Mix Lo-ROX

500

rxns

PB20.21-05

5 x 1ml

ABI 7500, ViiaTM; QuantStudio; IIumina Eco; Stratagene Mx3000, MX3005, Mx4000

2000 rxns

PB20.21-20

20 x 1ml

qPCRBIO Probe Mix Separate-ROX

500 

rxns

PB20.24-05

5 x 1ml

BioRad CFX96, CFX384, Chromo4TM, CFXconnectTM, MiniOpticonTM; Roche LightCylcer (2.0,1.5,480,1536, Nano); MJ Research OpticonTM OpticonTM2, Chroma 4; Corbett Rotor-Gene (3000,6200,62H0,6500,65H0,6600)

2000 rxns

PB20.24-20

20 x 1ml

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