Consumable



qPCRBIO SyGreen 1-Step kit
상세설명
제품용도 Real Time PCR Reagent
브랜드 PCR Biosystems
제품개요 하나의 PCR tube 내에서 cDNA 합성과 real time PCR이 가능한 제품
제품소개
qPCRBIO SyGreen 1-Step kit는 하나의 PCR tube 내에서 cDNA 합성과 real-time PCR이 가능하도록 최적화 시킨 제품입니다. 이 제품은 열에 안정한 modified MMLV reverse transcriptase와 hot start polymerase, dNTPs, buffer, MgCl2, intercalating dye를 최적의 조건으로 혼합 한 제품입니다. 본 제품은 2가지로 구성 되는데, qPCRBIO SyGreen 1-Step Detect kit는 low copy number target에 대한 매우 높은 민감도를 갖도록 디자인 되었고, qPCRBIO SyGreen 1-Step Go kit는 높은 template 농도로부터 빠르고 정확한 결과를 얻도록 하였습니다.
제품 특징
• qPCRBIO SyGreen 1-Step Detect: template amounts of 1pg – 10ng total RNA or >0.01pg mRNA per reaction
• qPCRBIO SyGreen 1-Step Go: template amounts of 10pg – 100ng total RNA or >0.01pg mRNA per reaction
• 매우 높은 민감도와 특이도
• 열에 안정한 modified MMLV RTase
• OneStep qRT-PCR

응용분야
• Absolute quantification
• Relative gene expression analysis
• Detection of extremely low copy number targets

보관온도
• -20℃
 
 Figure 1. Comparison of qPCRBIO SyGreen 1-Step Go (purple) against competitor Bioline (blue).
 

Shows amplification traces of the ACTG1 gene from a dilution series of total RNA extracted from mouse liver. Total RNA concentration varied from 25pg to 250ng per 20μl reaction. Cycling conditions were 45°C 10min for cDNA synthesis, followed by 95°C 2min hot start, then 45 cycles of 95°C 10sec, 60°C 10sec on Roche LC480. qPCRBIO SyGreen 1-Step Go had equal performance at high RNA concentrations and superior performance at lower RNA concentrations, displaying linear spacing between amplification curves, earlier amplification by 3–4 cycles, and lower prevalence of primer dimer.
 


   
 
  
Figure 2a. Comparison of qPCRBIO SyGreen 1-Step Detect (blue) and qPCRBIO SyGreen 1-Step Go (purple), showing ideal template ranges for each product. The ACTG1 gene was amplified from a dilution series of total RNA extracted from mouse liver. qPCRBIO SyGreen 1-Step Go (purple) shows efficient amplification of total RNA in the range 100pg to 100ng per reaction. qPCRBIO SyGreen 1-Step Detect (blue) shows efficient amplification of total RNA in the range 10pg to 10ng per reaction (figure 2a) on a Roche LC480.  Figure 2b. Comparison of qPCRBIO SyGreen 1-Step Detect (blue) and qPCRBIO SyGreen 1-Step Go (purple), showing ideal template ranges for each product. The ACTG1 gene was amplified from a dilution series of total RNA extracted from mouse liver. qPCRBIO SyGreen 1-Step Go (purple) shows efficient amplification of total RNA in the range 100pg to 100ng per reaction. Cycling conditions were 45°C 10min for cDNA synthesis, followed by 95°C 2min hot start, then 45 cycles of 95°C 10sec, 60°C 30sec, concluding with a melt analysis (figure 2b) on a Roche LC480.
주문정보    

Product Name

Pack Size

Cat No.

Presentation

Compatible Model

qPCRBIO SyGreen 1-Step Detect Hi-ROX

100 rxns

PB25.12-01

[1 x 1ml mix] & [1 x 200µl RTase]

ABI 7000,7300,7700,7900,7900HT StepOnePlusTM; StepOneTM; OpenArray PRISMTM Seqencing Detecion Series

300 rxns

PB25.12-03

[3 x 1ml mix] & [3 x 200µl RTase]

1200 rxns

PB25.12-12

[12 x 1ml mix] & [12 x 200µl RTase]

qPCRBIO SyGreen 1-Step Detect Lo-ROX

100 rxns

PB25.11-01

[1 x 1ml mix] & [1 x 200µl RTase]

ABI 7500, ViiaTM; QuantStudio; IIumina Eco; Stratagene Mx3000, MX3005, Mx4000

300 rxns

PB25.11-03

[3 x 1ml mix] & [3 x 200µl RTase]

1200 rxns

PB25.11-12

[12 x 1ml mix] & [12 x 200µl RTase]

qPCRBIO SyGreen 1-Step Go Hi-ROX

100 rxns

PB25.32-01

[1 x 1ml mix] & [1 x 200µl RTase Go]

ABI 7000,7300,7700,7900,7900HT StepOnePlusTM; StepOneTM; OpenArray PRISMTM Seqencing Detecion Series

300 rxns

PB25.32-03

[3 x 1ml mix] & [3 x 200µl RTase Go]

1200 rxns

PB25.32-12

[12 x 1ml mix] & [12 x 200µl RTase Go]

qPCRBIO SyGreen 1-Step Go Lo-ROX

100 rxns

PB25.31-01

[1 x 1ml mix] & [1 x 200µl RTase Go]

ABI 7500, ViiaTM; QuantStudio; IIumina Eco; Stratagene Mx3000, MX3005, Mx4000

300 rxns

PB25.31-03

[3 x 1ml mix] & [3 x 200µl RTase Go]

1200 rxns

PB25.31-12

[12 x 1ml mix] & [12 x 200µl RTase Go]

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