Consumable



UltraScript 2.0 Reverse Transcriptase
상세설명
제품용도 Reverse Transcriptase
브랜드 PCR Biosystems
제품개요 낮은 RNA 농도로부터 고수율의 cDNA를 합성하는 고온내열성을 갖춘 reverse transcripase

제품소개  

 

UltraScript 2.0 역전사 효소는 견고하고 고열 안정성을 지닌 변형된 MMLV 역전사 효소로서 GC가 풍부하고 낮은 농도의 템플릿을 포함하여 광범위한 RNA 샘플 유형으로부터 탁월한 cDNA 합성 속도와 높은 수율을 얻기 위해 설계되었습니다.

제품특징  

 

• Highly thermostable reverse transcriptase 55°C to 65°C and above
• Advanced RNase inhibitor
• High cDNA yields from as little as 20pg totalRNA
• Accurate reverse transcription of GC-rich and highly structured transcripts
• Sensitive detection of low copy number transcripts
• Reduced RNase H activity
• Advanced buffer chemistry including Mg and dNTPs
• Available as a stand-alone enzyme with buffer, a cDNA synthesis kit with premixed oligos  

     optimised for qPCR analysis, and a cDNA synthesis kit with separate oligos. 

응용분야

 

• cDNA synthesis for qPCR and PCR analysis, cloning, cDNA library preparation and

    Next Generation Sequencing
• Viral RNA targets
• miRNA targets
• Effcient synthesis from total RNA or poly(A)+ RNA

주문정보


 Product Name

 Pack Size

 Cat No.

 Presentation

UltraScript 2.0
cDNA Synthesis Kit

 25 reactions

 PB30.31-02

 [1 x 25μl UltraScript 2.0] & [1 x 100μl reaction mix]

 100 reactions

 PB30.31-10

 [1 x 100μl UltraScript 2.0] & [4 x 100μl reaction mix]

UltraScript 2.0
cDNA Synthesis Kit

Separate Oligos

 25 reactions

 PB30.32-02

 [1 x 25μl UltraScript 2.0] & [1 x 200μl buffer] & [1 x 100μl
Anchored Oligo(dT)18] & [1 x 100μl Random Hexamers]

 100 reactions

 PB30.32-10

 [1 x 100μl UltraScript 2.0] & [2 x 200μl buffer] & [1 x 100μl
Anchored Oligo(dT)18] & [1 x 100μl Random Hexamers]

UltraScript 2.0
Reverse Transcriptase

 10,000 units

 PB30.33-01

 [2 x 25μl UltraScript 2.0, 200 u/μl] & [1 x 200μl buffer]

 40,000 units

 PB30.33-04

 [2 x 100μl UltraScript 2.0, 200 u/μl] & [4 x 200μl buffer]

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