Consumable



VeriFi Polymerase
상세설명
제품용도 high fidelity PCR enzyme
브랜드 PCRbio systems
제품개요 더 높은 정확성이 요구되는 모든 PCR applications을 위해 설계된 high fidelity enzyme

 

제품 소개

 

PCRBIO VeriFi Polymerase는  cloning, site-directed mutagenesis 및 sequencing과 같은 더 높은 정확성이 요구되는 모든 PCR applications을 위해 설계된 high fidelity enzyme입니다.


이 enzyme은  3’-5’ exonuclease (proofreading) 활성을 위한  Pfu DNA polymerase 로부터 유래한 것입니다.

또한 dNTP, Mg 및 enhancer가 포함된 고급 buffer system이 제공되어 광범위한 target의 high fidelity PCR이 가능합니다.

 

제품 특징

 

Taq DNA 중합 효소보다 100 배 이상 높은 충실도

17.5kb 이상의 복잡한 genomic templates 에서도 증가된 PCR 성공률을 보임

GC and AT rich sequence를 포함하여 까다로운 템플릿으로부터 효율적으로 증폭 가능

표준 PCR 조건 (10-30 초 / kb)에서 높은 수율

GC bias감소를 위한 고온(~100°C) 사이클링

 

Application

Long range PCR

High fidelity PCR

Site-directed mutagenesis

Cloning

Next Generation Sequencing

 

  

 

 Figure 1. Versatility across a broad range of amplicon lengths

 PCRBIO VeriFi Polymerase amplifies the range of fragment lengths indicated with high yield and specificity. 

 The starting template amount is 4-30ng of mouse or human genomic DNA, diluted 1.5 to 3 fold. GC content ranges from 37-55%. 

 L: PCRBIO Ladder II.

 


 

 Figure 2. Increased success rates with complex templates

 Amplification of a 17.5kb fragment of the HBB gene. 

 The starting template amount is 150ng (A and C) and 30ng (B and D) of human genomic DNA, diluted 2 fold. 

 A 2-step PCR protocol was used with amplification at 72°C (A and B) or 68°C (C and D). GC content is 37%. 

 PCRBIO VeriFi   Polymerase amplifies long fragments with yields comparable to T-company DNA Polymerase. L: PCRBIO Ladder II. 

 


 

 Figure 3. Amplification of targets with high sensitivity and specificity compared to leading competitors 

 Amplification of a 1.0kb fragment of the GAPDH gene with different starting template amounts of mouse genomic DNA. 

 A: 20ng, B: 3.2ng, C: 0.5ng, D: 0.08ng. GC content is 51%. L: PCRBIO Ladder IV. 

 The reactions were set up following manufacturers’ recommendations. 

 Cycling conditions were 95°C 2 min, then 30 cycles of 98°C 15 sec, 66°C 15 sec and 72°C 30 sec. 

 PCRBIO VeriFi Polymerase displays greater sensitivity and specificity compared to leading competitors. 

 

 

주문정보

 


 

 Cat. No.

 Product Name 

 Pack Size 

 Presentation

 PB10.42-01

 PCRBIO VeriFi Polymerase

 100 Units

 [1 x 0.05mL 2u/µL] & [1 x 1.7mL buffer]          

 PB10.42-05

 

 500 Units

 [1 x 0.250mL 2u/µL] & [3 x 1.7mL buffer]

 PB10.43-01

 PCRBIO VeriFi Mix

 100 x 50µL Reactions          

 2 x 1.25mL

 PB10.43-05

 

 500 x 50µL Reactions

 2 x (5 x 1.25mL)

 PB10.44-01

 PCRBIO VeriFi Mix Red

 100 x 50µL Reactions

 2 x 1.25mL

 PB10.44-05

 

 500 x 50µL Reactions

 2 x (5 x 1.25mL)

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