Consumable



qPCRBIO HRM Mix
상세설명
제품용도 Real Time PCR Reagent
브랜드 PCR Biosystems
제품개요 mutation 및 SNP와 methlylation 분석을 위한 3세대의 saturating dye를 사용한 HRM Mix

제품소개
High Resolution Melt 분석은 이중가닥의 DNA 샘플에서 mutation, SNP genotyping이나 methylation 분석 등을 할 수 있는 기술입니다.
 

qPCRBIO HRM Mix는 hot start polymerase, dNTPs, buffer, Mgcl2와 double strand 상태의 DNA에 대한 감도가 높은 SyGreen II dye를 혼합해 높은 형태로, HRM 기능이 있는 thermal cycler에서 사용이 가능한 제품입니다. 

제품 특징
• 3세대 saturating dye SyGreen II 혼합
• Antibody-mediated hot start

응용분야
• SNP genotyping
• Gene scanning
• CpG methylation analysis

보관온도
• -20℃

 

 
   
Figure 1 & 2    Figure 3 & 4 
HRM analysis of class I, II, III and IV single nucleotide polymorphisms. In each case, three samples of human genomic DNA were amplified using qPCRBIO HRM Mix with primers specific for a fragment of gene containing the SNPs. Figure 1 shows Factor V 1691G/A (class I), figure 2 shows MTHFR 1298A/C (class II) After amplification the products were subjected to HRM analysis. The traces show clear allele calling of class I, II, III and IV SNPs.   HRM analysis of class I, II, III and IV single nucleotide polymorphisms. In each case, three samples of human genomic DNA were amplified using qPCRBIO HRM Mix with primers specific for a fragment of gene containing the SNPs. figure 3 shows HFE 187 C/G (class III) and figure 4 shows beta-globulin 17A/T (class IV). After amplification the products were subjected to HRM analysis. The traces show clear allele calling of class I, II, III and IV SNPs.

 

주문정보   
Product Name

Pack Size

Cat No.

Presentations

qPCRBIO HRM Mix

100 rxns

PB20.31-01

1 x 1ml

500 rxns

PB20.31-05

5 x 1ml

2000 rxns

PB20.31-20

20 x 1ml

 

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