Consumable



제품소개
qPCRBIO Probe 1-Step Go kit는 하나의 PCR tube 내에서 cDNA 합성과 real-time PCR이 가능하도록 최적화 시킨 제품입니다. 이 제품은 열에 안정한 modified MMLV reverse transcriptase와 hot start polymerase, dNTPs, buffer, MgCl2를 최적의 조건으로 혼합 한 제품입니다. 본 제품은 sequence specific probe에 적합한 제품으로 모든 real-time PCR 기기에 사용이 가능합니다.
제품 특징
• High efficiency in multiplex reactions
• 열에 안정한 modified MMLV RTase
• 매우 높은 민감도와 특이도
• OneStep qRT-PCR

응용분야
• Absolute quantification
• Relative gene expression analysis
• TaqMan, Scorpions and molecular beacon probes
• Detection of extremely low copy number targets
• Multiplex or singleplex
• Diagnostic real-time PCR

보관온도
• -20℃




  

Figure 1. High efficiency and broad dynamic range. Cycling conditions: 45°C 10min, 95°C 3min, then 45 cycles of 95°C 10sec, 60°C 30sec. Shows TaqMan probe amplification traces of mouse gene ACTB using mouse liver total RNA as template in triplicate. Template concentrations are 10x serial dilutions ranging from 10pg to 1μg total RNA per 20μl reaction. qPCRBIO Probe 1-Step Go shows high efficiency over a broad dynamic range. 

 

 

 

 
Figure 2. qPCRBIO Probe 1-Step Go in multiplex
Four mouse housekeeping genes were amplified simultaneously in a single multiplex reaction.
1μg of mouse liver total RNA was used as template. Amplification was detected using TaqMan® probes in the following gene/probe combinations: B2MG/HEX, ACTB/ Cy5, GAPDH/FAM, and ACTG/TexasRed. Cycling conditions were 45°C 10min, 95°C 3min, then 45 cycles of 95°C 10s, 60°C 30s. This demonstrates that the qPCRBIO Probe 1-Step Go mix can be used to quantify and compare expression levels of multiple genes in a single reaction.

 

주문정보    

Product Name

Pack Size

Cat No.

Presentation

Compatible Model

qPCRBIO Probe 1-Step Go Hi-ROX

100 rxns

PB25.42-01

[1 x 1ml mix] & [1 x 100µl RTase Go]

ABI 7000,7300,7700,7900,7900HT StepOnePlusTM; StepOneTM; OpenArray PRISMTM Seqencing Detecion Series

300 rxns

PB25.42-03

[3 x 1ml mix] & [3 x 100µl RTase Go]

1200 rxns

PB25.42-12

[12 x 1ml mix] & [12 x 100µl RTase Go]

qPCRBIO Probe 1-Step Go Lo-ROX

100 rxns

PB25.41-01

[1 x 1ml mix] & [1 x 100µl RTase Go]

ABI 7500, ViiaTM; QuantStudio; IIumina Eco; Stratagene Mx3000, MX3005, Mx4000

300 rxns

PB25.41-03

[3 x 1ml mix] & [3 x 100µl RTase Go]

1200 rxns

PB25.41-12

[12 x 1ml mix] & [12 x 100µl RTase Go]

qPCRBIO Probe 1-Step Go No-ROX

100 rxns

PB25.43-01

[1 x 1ml mix] & [1 x 100µl RTase Go]

BioRad CFX96, CFX384, Chromo4TM, CFXconnectTM, MiniOpticonTM; Roche LightCylcer (2.0,1.5,480,1536, Nano); MJ Research OpticonTM OpticonTM2, Chroma 4; Corbett Rotor-Gene

300 rxns

PB25.43-03

[3 x 1ml mix] & [3 x 100µl RTase Go]

1200 rxns

PB25.43-12

[12 x 1ml mix] & [12 x 100µl RTase Go]

qPCRBIO Probe 1-Step Hi-ROX

100 rxns

PB25.22-01

[1 x 1ml mix] & [1 x 200µl RTase]

ABI 7000,7300,7700,7900,7900HT StepOnePlusTM; StepOneTM; OpenArray PRISMTM Seqencing Detecion Series

300 rxns

PB25.22-03

[3 x 1ml mix] & [3 x 200µl RTase]

1200 rxns

PB25.22-12

[12 x 1ml mix] & [12 x 200µl RTase]

qPCRBIO Probe 1-Step Lo-ROX

100 rxns

PB25.21-01

[1 x 1ml mix] & [1 x 200µl RTase]

ABI 7500, ViiaTM; QuantStudio; IIumina Eco; Stratagene Mx3000, MX3005, Mx4000

300 rxns

PB25.21-03

[3 x 1ml mix] & [3 x 200µl RTase]

1200 rxns

PB25.21-12

[12 x 1ml mix] & [12 x 200µl RTase]

qPCRBIO Probe 1-Step No-ROX

100 rxns

PB25.23-01

[1 x 1ml mix] & [1 x 200µl RTase]

BioRad CFX96, CFX384, Chromo4TM, CFXconnectTM, MiniOpticonTM; Roche LightCylcer (2.0,1.5,480,1536, Nano); MJ Research OpticonTM OpticonTM2, Chroma 4; Corbett Rotor-Gene

300 rxns

PB25.23-03

[3 x 1ml mix] & [3 x 200µl RTase]

1200 rxns

PB25.23-12

[12 x 1ml mix] & [12 x 200µl RTase]

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